Publication: Immunohistochemical analyses
on albumin and immunoglobulin in acute
hypertensive mouse kidneys by “in vivo cryotechnique”
Authors
Li, Z. ; Terada, N. ; Ohno, N. ; Ohno, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The purpose of this study is to visualize
topographical changes of serum proteins, albumin and
immunoglobulin, passing through mouse glomerular
capillary loops and their reabsorption in renal proximal
tubules by immunohistochemistry in combination with
our “in vivo cryotechnique”. The “in vivo
cryotechnique” was performed on left mouse kidneys
under normotensive, experimentally acute hypertensive
and heart-arrest conditions. The cryofixed tissues by the
technique were routinely processed for freezesubstitution.
Serial deparaffinized sections were stained
with hematoxylin-eosine and immunostained with antimouse
albumin, immunoglobulin G (IgG), kappa or
lambda light chain and IgG1 heavy chain antibodies.
Under the normotensive and heart-arrest conditions,
albumin and IgG were clearly immunolocalized in blood
vessels and slightly in apical cytoplasmic parts of some
proximal tubules. Under the acute hypertensive
condition, the albumin and kappa or lambda light chains,
but not IgG1 heavy chain, were strongly
immunolocalized in the apical cytoplasm of almost all
proximal tubules. This study is the first in vivo
visualization for glomerular passage of serum proteins
and their transtubular absorption. Thus, the “in vivo
cryotechnique” with freeze-substitution can be used for
clarifying not only the functional morphology of living
animal cells, but also in situ immunohistochemical
localization of their components.
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