Publication: Tracing the accumulation and effects of mercury uptake in the previtellogenic ovary of crucian carp Carassius auratus gibelio by autometallography and
caspase-3 immunohistochemistry
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Date
2009
Authors
Zarnescu, Otilia
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The aims of the present study were to apply
the AMG technique for localization of mercury at the
light and electron microscopic level in the ovary of
crucian carp after exposure to mercuric chloride and to
find out if this heavy metal induces expression of
caspase-3. Depending on the stage of ovarian follicle
development, two patterns of mercury accumulation
have been found in previtellogenic ovary of crucian
carp. The first mercury accumulation pattern has been
found in the early previtellogenic oocyte without zona
radiata. In these oocytes, mercury accumulates into an
ooplasmic region that seems to correspond to the
Balbiani body (32-65 μm oocyte diameter), throughout
the cytoplasm (84-116 μm oocyte diameter) and in the
cortical cytoplasm (~180 μm oocyte diameter). The
second mercury accumulation pattern has been found in
the late previtellogenic oocyte with cortical alveoli (229-
330 μm oocyte diameter). Ultrastructural observations
have shown grains of silver-enhanced mercury inside
coated vesicles, the cortical lysosome-like bodies or
multivesicular bodies and cortical alveoli. Immunohistochemistry
reaction for caspase-3 was positive in
nuclei of the early previtellogenic oocyte and Balbiani
body.
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