Publication: Effect of superovulation treatment on oocyte´s DNA methylation.
Authors
Lopes, J.S. ; Ivanova, E. ; Ruiz, S. ; Andrews, S. ; Kelsey, G. ; Coy, P.
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Publisher
MDPI
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DOI
https://doi.org/10.3390/ijms232416158
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info:eu-repo/semantics/article
Description
© 2022. The authors. This document is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/
This document is the accepted version of a published work that appeared in final form in
International Journal of Molecular Sciences
Abstract
Controlled ovarian stimulation is a necessary step in some assisted reproductive procedures
allowing a higher collection of female gametes. However, consequences of this stimulation for the
gamete or the offspring have been shown in several mammals. Most studies used comparisons
between oocytes from different donors, which may contribute to different responses. In this work,
we use the bovine model in which each animal serves as its own control. DNA methylation profiles
were obtained by single-cell whole-genome bisulfite sequencing of oocytes from pre-ovulatory
unstimulated follicles compared to oocytes from stimulated follicles. Results show that the global
percentage of methylation was similar between groups, but the percentage of methylation was lower
for non-stimulated oocytes in the imprinted genes APEG3, MEG3, and MEG9 and higher in TSSC4
when compared to stimulated oocytes. Differences were also found in CGI of imprinted genes:
higher methylation was found among non-stimulated oocytes in MEST (PEG1), IGF2R, GNAS (SCG6),
KvDMR1 ICR UMD, and IGF2. In another region around IGF2, the methylation percentage was
lower for non-stimulated oocytes when compared to stimulated oocytes. Data drawn from this study
might help to understand the molecular reasons for the appearance of certain syndromes in assisted
reproductive technologies-derived offspring.
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Citation
International Journal of Molecular Sciences 2022, 23(24), 16158
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