Oleanolic acid induces migration in Mv1Lu and MDA-MB-231 epithelial cells involving EGF receptor and MAP kinases activation

Bernabé García, Ángel; Armero Barranco, David; Liarte, Sergio; Ruzafa Martínez, María; Ramos Morcillo, Antonio Jesús; Nicolás, Francisco José

Publication:
Oleanolic acid induces migration in Mv1Lu and MDA-MB-231 epithelial cells involving EGF receptor and MAP kinases activation

dc.contributor.author	Bernabé García, Ángel
dc.contributor.author	Armero Barranco, David
dc.contributor.author	Liarte, Sergio
dc.contributor.author	Ruzafa Martínez, María
dc.contributor.author	Ramos Morcillo, Antonio Jesús
dc.contributor.author	Nicolás, Francisco José
dc.contributor.department	Enfermería
dc.date.accessioned	2024-02-28T11:37:09Z
dc.date.available	2024-02-28T11:37:09Z
dc.date.issued	2017-02-23
dc.description	©2017. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published, version of a Published Work that appeared in final form in PLoS ONE. To access the final edited and published work see https://doi.org/10.1371/journal.pone.0172574	es
dc.description.abstract	During wound healing, skin function is restored by the action of several cell types that undergo differentiation, migration, proliferation and/or apoptosis. These dynamics are tightly regulated by the evolution of the extra cellular matrix (ECM) contents along the process. Pharmacologically active flavonoids have shown to exhibit useful physiological properties interesting in pathological states. Among them, oleanolic acid (OA), a pentacyclic triterpene, shows promising properties over wound healing, as increased cell migration in vitro and improved wound resolution in vivo. In this paper, we pursued to disclose the molecular mechanisms underlying those effects, by using an in vitro scratch assay in two epithelial cell lines of different linage: non-malignant mink lung epithelial cells, Mv1Lu; and human breast cancer cells, MDA-MB-231. In every case, we observed that OA clearly enhanced cell migration for in vitro scratch closure. This correlated with the stimulation of molecular pathways related to mitogen-activated protein (MAP) kinases, as ERK1,2 and Jun N-terminal kinase (JNK) 1,2 activation and c-Jun phosphorylation. Moreover, MDA-MB-231 cells treated with OA displayed an altered gene expression profile affecting transcription factor genes (c-JUN) as well as proteins involved in migration and ECM dynamics (PAI1), in line with the development of an epithelial to mesenchymal transition (EMT) status. Strikingly, upon OA treatment, we observed changes in the epidermal growth factor receptor (EGFR) subcellular localization, while interfering with its signalling completely prevented migration effects. This data provides a physiological framework supporting the notion that lipophilic plant extracts used in traditional medicine, might modulate wound healing processes in vivo through its OA contents. The molecular implications of these observations are discussed.	es
dc.format	application/pdf	es
dc.format.extent	24	es
dc.identifier.citation	PLoS ONE 12(2) 2017: e0172574
dc.identifier.doi	https://doi.org/10.1371/journal.pone.0172574
dc.identifier.issn	Electronic: 1932-6203
dc.identifier.uri	http://hdl.handle.net/10201/139708
dc.language	eng	es
dc.publisher	Public Library of Science	es
dc.relation	Sin financiación externa a la Universidad	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject.other	CDU::6 - Ciencias aplicadas::61 - Medicina::614 - Higiene y salud pública. Contaminación. Prevención de accidentes. Enfermería	es
dc.title	Oleanolic acid induces migration in Mv1Lu and MDA-MB-231 epithelial cells involving EGF receptor and MAP kinases activation	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es