Publication: Claudin-7 protein differentiates canine cholangiocarcinoma from hepatocellular carcinoma
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Date
2010
Authors
Jakab, Cs. ; Kiss, A. ; Schaff, Z. ; Szabó, Z. ; Rusvai, M. ; Gálfi, P. ; Szabára, Á. ; Sterczer, A. ; Kulka, J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The aim of the present study was to
characterise the expression pattern of claudin-7 tight
junction protein in canine normal liver, hyperplastic and
primary neoplastic lesions of the canine liver and
whether this tight junction protein can help differentiate
canine cholangiocarcinomas from canine hepatocellular
carcinomas. Methods and results: Necropsy samples
included 15 canine normal liver tissue samples, 10
hepatocellular nodular hyperplasias, 6 hepatocellular
adenomas, 15 well-differentiated and 6 poorly
differentiated hepatocellular carcinomas, 6
cholangiocellular hyperplasias, 10 cholangiocellular
adenomas, 15 well-differentiated and 6 poorly
differentiated cholangiocarcinomas, 6 normal
extrahepatic bile ducts, 8 normal gall bladder tissue
samples, and 5 cystic mucinous hyperplasias of the gall
bladder. In all canine normal liver tissue samples the
hepatocytes were negative for claudin-7 and the normal
biliary epithelial cells showed intense basolateral
membrane claudin-7 positivity. In all cholangiocellular
hyperplasia samples and in all cholangiocellular
adenoma samples the benign cholangiocytes showed
intense basolateral membrane positivity for claudin-7. In
all samples of the well-differentiated and poorly
differentiated cholangiocarcinomas, the malignant
neoplastic biliary epithelial cells showed intense
basolateral membrane positivity for claudin-7. Neither
the hyperplastic nodules of the liver cells nor the
hepatocellular adenomas reacted with claudin-7. The
well-differentiated and poorly differentiated
hepatocellular cancers were negative for claudin-7. The epithelial cells of canine normal extrahepatic bile ducts,
gall bladder and cystic mucinous hyperplasias of the gall
bladder showed intense basolateral membrane positivity
for claudin-7. Differences in the intensity of claudin-7
reaction were not apparent among different types of
proliferative lesions of cholangiocytes or degrees of
cellular differentiation of neoplastic biliary epithelial
cells.
Conclusion: Consequently, we hypothesize that
claudin-7 is an excellent immunohistochemical marker
of the cholangiocellular differentiation in canines and
can be used to detect benign and malignant proliferative
lesions of the canine biliary tract. It can also help to
differentiate canine cholangiocarcinomas from
hepatocellular carcinomas.
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