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Seminal plasma cytokines are predictive of the outcome of boar sperm preservation

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dc.contributor.authorBarranco, Isabel
dc.contributor.authorPadilla, Lorena
dc.contributor.authorPerez-Patiño, Cristina
dc.contributor.authorVazquez, Juan M
dc.contributor.authorMartinez, Emilio A
dc.contributor.authorRodriguez-Martinez, Heriberto
dc.contributor.authorRoca, Jordi
dc.contributor.authorParrilla, Inmaculada
dc.contributor.departmentMedicina y Cirugía Animal
dc.date.accessioned2024-02-02T12:52:14Z
dc.date.available2024-02-02T12:52:14Z
dc.date.issued2019-12-04
dc.description©2019. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/ This document is the Published, version of a Published Work that appeared in final form in Frontiers in Veterinary Science. To access the final edited and published work see https://doi.org/10.3389/fvets.2019.00436
dc.description.abstractBackground: Boar seminal plasma is rich in cytokines, which could influence the capability of spermatozoa to tolerate preservation. Objectives: To evaluate the involvement of boar seminal plasma cytokines in the changes experienced by boar spermatozoa during their storage, either in liquid or frozen state. Materials and Methods: In two separated experiments, semen samples from healthy and fertile boars were split in two aliquots, one centrifuged twice (1,500 ×g for 10 min) to harvest seminal plasma, whereas the other was either commercially extended (3 × 107 sperm/mL) and liquid-stored at 17°C during 144 h (n = 28, Experiment 1) or frozen-thawed using a standard 0.5 mL protocol (n = 27, Experiment 2). Sixteen cytokines were quantified using Luminex xMAP®. Sperm attributes (CASA-evaluated total and progressive motility; flow cytometry-evaluated sperm viability, production of intracellular H2O2 and O∙−2 and levels of lipid peroxidation in viable spermatozoa) were evaluated either at 0, 72, or 144 h of liquid storage (Experiment 1) or before freezing and at 30- and 150-min post-thawing (Experiment 2). Results: Multiple linear regression models, with Bayesian approach for variable selection, revealed that the anti-inflammatory TGF-β2, TGF-β3, IL-1Ra, and IL-4 and the pro-inflammatory IL-8 and IL-18, predicted changes in sperm motility for liquid-stored semen while the anti-inflammatory IFN-γ was included in the models predicting changes in all sperm attributes for cryopreserved semen. Conclusion: Specific boar seminal plasma cytokines would contribute to modulate the structural and metabolic changes shown by spermatozoa during preservation, either in liquid or frozen state.es
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dc.format.extent10
dc.identifier.citationFrontiers in Veterinary Science 2019. 6:436.
dc.identifier.doihttps://doi.org/10.3389/fvets.2019.00436
dc.identifier.urihttp://hdl.handle.net/10201/138551
dc.languageenges
dc.relationMINECO and FEDER [AGL2015-69738-R] Madrid (Spain), Seneca Foundation [19892/GERM/15] Murcia (Spain), Swedish Research Council Formas [2017-00946], Swedish Research Council [Vetenskapsrådet, VR, 2015-05919], and FORSS [745971, Stockholm, Sweden]es
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fvets.2019.00436/fulles
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectSeminal plasmaes
dc.subjectCytokines
dc.subjectSpermatozoa
dc.subjectLiquid storage
dc.subjectCryopreservation
dc.subjectPig
dc.titleSeminal plasma cytokines are predictive of the outcome of boar sperm preservationes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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