Publication: Histoenzymological detection of sialic acids in the rodent salivary glands
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Date
1996
Authors
Accili, Daniela ; Gabrielli, M.G. ; Menghi, Giovanna ; Materazzi, G.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Sections from the major salivary glands of
rats and mice were used to locate, charactecize and
compare sialoglycoconjugates by means of lectin
histochemistry, sialidase digestion, periodate oxidation
and potassium hydroxide deacetylation. The gland
sialylated macromolecules contained the terminal dimers
sialic acid-B-galactose and sialic acid-a-N-acetylgalactosamine
but differed in the varieties of sialic acids
and the linkages of sialic acids to penultimate sugars.
Indeed, the submandibular and parotid glands exhibited
a notable occurrence of periodate labile sialic acids with
C7 andlor C8 andlor C9 acetyl groups in their
polyhydroxyl chains. In particular, C9 acetylated sialic
acids were mostly linked a2-6 to B-galactose. The
sublingual glands, instead, were strongly characterized
by a presence of C9 acetylated sialic acids bound a2-3 to
B-galactose. Also, sialic acids with O-acetyl substituents
at C4 were evident in the mouse parotid gland and in the
rat submandibular and sublingual glands. The great
variety of sialoderivatives expressed by the rodent
salivary glands was correlated with the differential
involvement of these compounds in lubricating and
defensive processes. Sex-related differences regarding
the sialic acid location, acetylation degree and linkage
were shown in the submandibular glands of both species.
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