Publication: Morphological study of erythrocyte shapes in red pulp of mouse spleens revealed by an in vivo cryotechnique
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Date
2001
Authors
Xue, M. ; Baba, T. ; Terada, N. ; Kato, Y. ; Fuji, Y. ; Ohno, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The purpose of this study was to clarify
erythrocyte shapes in splenic cords of living mouse
spleens, using our in vivo cryotechnique followed by
scanning (SEM) or transmission (TEM) electron
microscopy. Some spleens of mice were quickly frozen
by the in vivo cryotechnique while their hearts were
beating under anesthesia. In contrast, other spleens were
prepared by an in vitro freezing method after they were
taken out from the abdominal cavity. They were
routinely freeze-substituted, and prepared for SEM and
TEM. A few mouse spleens were also routinely fixed
and embedded in Quetol-812 to obtain conventional
morphology. Erythrocytes in living mouse spleens
showed a variety of shapes with narrow spaces between
them, trapped among reticular fiber tissues. Similar
various shapes of erythrocytes were kept in the red pulp
even after blocking normal blood circulation, as
prepared by the in vitro freezing method. In comparison
to the above-mentioned findings, some erythrocytes
were changed to biconcave discoid shapes by the
conventional immersion fixation with chemical fixatives.
They also showed wide spaces between each other
among reticular fiber tissues. Such conventional
morphological studies could hardly reveal the in vivo
shapes of erythrocytes in functioning spleens with
normal blood circulation. In contrast, the various shapes
of erythrocytes in the functioning spleens were
demonstrated by our in vivo cryotechnique. It is
suggested that most erythrocytes congesting in spleens keep their original configuration in spite of
microenviromental alteration in splenic blood
circulation.
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