Publication: Expression of major histocompatibility complex
antigens and CR3 complement receptors in activated
microglia following an injection of ricin into the sciatic
nerve in rats
Authors
Ling, E. A. ; Kaur, C. ; Wong, W. C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The ventral horn motor neurons in the
lower lumbar cord underwent rapid degeneration
following an injection of Ricinus communis agglutinin-
60 (RCA) into the sciatic nerve. The cell death which
was most drastic between the fifth and seventh postinjection
day elicited a significant increase in the
number of microglia. The activated microglia were
scattered throughout the neuropil but the dramatic
feature was their close association with the somata of
the degenerating neurons. Often several microglial
cells were seen surrounding the soma of a
degenerating neuron. Immunocytochemical study
showed that both the interstitial as well as the
perineuronal activated microglia were labelled with the
monoclonal antibodies OX-18 and OX-42 for the
detection of MHCI encoded antigen and type three
complement receptors, respectively. Intense
immunoreactivity was observed especially in the
perineuronal microglia with OX-18. Electron
microscopic study confirmed the identification of the
activated microglia. Although the activated microglia
closely apposed the neuronal soma, there was no sign
of a direct endocytosis. The cytoplasm of the activated
microglia, however, contained massive lipofuscin
bodies in longer survival animals. Electron microscopic
immunocytochemical study showed that the
immunoreactivity of the activated microglia was
localized along their plasma membrane facing the
neuronal soma. Since the microglia cells on the
contralateral side of the ventral horn were not marked
by the antibodies used, it was postulated that the
vigorous expression of MHCI antigen and CR3
receptors on the activated microglia was induced by
the neuronal degeneration resulting from the
application of the toxin ricin.
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