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MiR-5195-3p functions as a tumor suppressor by targeting RHBDD1 in ovarian cancer

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dc.contributor.authorWang, Zhanyu
dc.contributor.authorZhang, Xiaoping
dc.contributor.authorLiu, Yongying
dc.contributor.authorShi, Xiaoyan
dc.contributor.authorLi, Lijun
dc.contributor.authorJia, Yun
dc.contributor.authorWu, Fangfang
dc.contributor.authorCui, Haosen
dc.contributor.authorLi, Liang
dc.date.accessioned2023-12-01T09:17:02Z
dc.date.available2023-12-01T09:17:02Z
dc.date.issued2023
dc.description.abstractBackground. Recent studies have reported the tumor suppressive role of miR-5195-3p in the progression of several cancers, but the potential roles of miR-5195-3p in ovarian cancer (OC) remain largely unknown. Methods. We first analyzed the expression levels of miR-5195-3p in 83 pairs of human OC tissues and adjacent specimens by reverse transcription-quantitative PCR. The correlation of miR-5195-3p/rhomboid domain containing 1 (RHBDD1) and clinicopathological parameters was analyzed by chi-square test. The prognostic value of miR-5195-3p was evaluated by Kaplan-Meier method Cox proportional hazards models. The effects of miR-5195-3p on cell proliferation, cell cycle distribution, migration and invasion were examined by CCK-8 assay, colony formation assay, flow cytometry and transwell assay. Tumor forming was evaluated by nude mice model in vivo. The association between miR-5195-3p and RHBDD1 was verified by luciferase reporter assay. Results. We observed that miR-5195-3p level was remarkably reduced in OC tissues as compared to adjacent tissues. The expression of miR-5195-3p was associated with FIGO stage, depth of invasion and poor survival prognosis in OC patients. Overexpression of miR-5195-3p significantly suppressed cell proliferation, cell cycle G1/S transition, migration and invasion in OC cell lines (SKOV-3 and OVCAR3), while knockdown of miR-5195-3p obtained the opposite results. We further confirmed miR-5195-3p as a negative posttranscriptional modulator of RHBDD1. RHBDD1 expression was upregulated in OC tissues compared with adjacent tissues, which was inversely correlated with miR-5195-3p expression. The expression of RHBDD1 was associated with FIGO stage and distant metastasis. RHBDD1 overexpression reversed the suppressive role of miR-5195-3p on OC cell proliferation, migration and invasion. Consistent with the in vitro results, miR-51953p overexpression decreased the growth of subcutaneously inoculated tumors in nude mice. Conclusions. Taken together, the present results indicated that miR-5195-3p acts a tumor suppressor by targeting RHBDD1 in OC.es
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dc.format.extent11es
dc.identifier.citationHistology and Histopathology Vol. 38, nº12 (2023)
dc.identifier.doihttps://doi.org/10.14670/HH-18-595
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/136272
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectOvarian canceres
dc.subjectmiR-5195-3pes
dc.subjectRHBDD1es
dc.subjectPrognosises
dc.subjectCell functionses
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleMiR-5195-3p functions as a tumor suppressor by targeting RHBDD1 in ovarian canceres
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.38,nº12 (2023)