Quantification of photoreceptors’ changes in a diabetic  retinopathy model with two-photon imaging microscopy

Bautista-Elivar, Nazario; Avilés Trigueros, Marcelino; Bueno García, Juan Manuel

Publication:
Quantification of photoreceptors’ changes in a diabetic retinopathy model with two-photon imaging microscopy

dc.contributor.author	Bautista-Elivar, Nazario
dc.contributor.author	Avilés Trigueros, Marcelino
dc.contributor.author	Bueno García, Juan Manuel
dc.contributor.department	Oftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
dc.contributor.other	Facultad de Óptica y Optometría
dc.date.accessioned	2025-02-02T16:15:54Z
dc.date.available	2025-02-02T16:15:54Z
dc.date.copyright	© 2024 The authors
dc.date.issued	2024-08-11
dc.description.abstract	Emerging evidence suggests that retinal neurodegeneration is an early event in the pathogenesis of diabetic retinopathy (DR), preceding the development of microvascular abnormalities. Here, we assessed the impact of neuroinflammation on the retina of diabetic-induced rats. For this aim we have used a two-photon microscope to image the photoreceptors (PRs) at different eccentricities in unstained retinas obtained from both control (N = 4) and pathological rats (N = 4). This technique provides high-resolution images where individual PRs can be identified. Within each image, every PR was located, and its transversal area was measured and used as an objective parameter of neuroinflammation. In control samples, the size of the PRs hardly changed with retinal eccentricity. On the opposite end, diabetic retinas presented larger PR transversal sections. The ratio of PRs suffering from neuroinflammation was not uniform across the retina. Moreover, the maximum anatomical resolving power (in cycles/deg) was also calculated. This presents a double-slope pattern (from the central retina towards the periphery) in both types of specimens, although the values for diabetic retinas were significantly lower across all retinal locations. The results show that chronic retinal inflammation due to diabetes leads to an increase in PR transversal size. These changes are not uniform and depend on the retinal location. Two-photon microscopy is a useful tool to accurately characterize and quantify PR inflammatory processes and retinal alterations.
dc.format	application/pdf	es
dc.format.extent	12	es
dc.identifier.citation	Int. J. Mol. Sci. 2024, 25, 8756
dc.identifier.doi	https://doi.org/10.3390/ijms25168756
dc.identifier.issn	Print: 1661-6596
dc.identifier.issn	Electronic: 1422-0067
dc.identifier.uri	http://hdl.handle.net/10201/149963
dc.language	eng	es
dc.publisher	MDPI	es
dc.relation	This research was funded by Agencia Estatal de Investigación, Spain (grant PID2020-113919RB-I00/AEI/10.13039/501100011033).	es
dc.relation.publisherversion	https://www.mdpi.com/1422-0067/25/16/8756	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Atribución 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	Two-photon microscopy	es
dc.subject	Diabetic retinopathy	es
dc.subject	Neuroinflammation	es
dc.subject	Photoreceptors	es
dc.title	Quantification of photoreceptors’ changes in a diabetic retinopathy model with two-photon imaging microscopy	es
dc.type	info:eu-repo/semantics/article	es
dc.type.version	info:eu-repo/semantics/PublishedVersión
dspace.entity.type	Publication	es
relation.isAuthorOfPublication	03e0f62a-075d-49de-ac29-6b4c980af0b1
relation.isAuthorOfPublication	460f1110-b358-474a-9e08-f92fabe4be2b
relation.isAuthorOfPublication.latestForDiscovery	03e0f62a-075d-49de-ac29-6b4c980af0b1