Publication: Human basophil recovery from secretion. A review emphasizing the distribution of Charcot-Leyden crystal protein in cells stained with the postf ixation electron-dense tracer, cationized ferritin
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Date
1996
Authors
Dvorak, A. M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Basophils of two species, guinea pigs and
humans, have been shown by ultrastructural analyses to
recover from noncytotoxic secretory processes by
conservation and synthetic mechanisms. In human
basophils, an electron-dense tracer (cationized femtin)
used after fixation labels membranes in continuity with
plasma membranes at planes of section out of view,
thereby indicating internalization of previously
externalized granule membranes. Conservation of
previously emptied granule containers that remain in the
cytoplasm after secretion was associated with
reaccumulation of electron-dense particles and
condensation of these dense materials therein. Granuleand
vesicle-poor, previously stimulated cells developed
large numbers of cytoplasmic vesicles beneath plasma
membranes, in expanding Golgi areas and in perigranular
areas of the cytoplasm. Cytochemical labeling
techniques to localize histamine and Charcot-Leyden
crystal protein revealed reaccumulation of these two
granule proteins in recovering human basophil granules.
The mechanism(s) of their recovery likely involves both
synthesis of new proteins and conservation by
internalization of secreted proteins bound to cell
surfaces.
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