Publication: Morphogenesis of rat experimental pulmonary emphysema induced by intratracheally administered papain: changes in elastic fibres
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Date
2006
Authors
Sánchez-Gascón, F. ; Girona, J.C. ; Bernal-Mañas, C.M. ; Morales, E. ; Canteras, M. ; Beltrán Frutos, Ester ; Pastor García, Luis Miguel
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The ultrastructural changes of elastic fibres
in emphysematous lungs have been studied in men, but
few works exist on this topic in experimental
emphysematous animals. In this paper, the
morphogenesis of emphysema and alterations of the
elastic fibres produced by the instillation of papain are
described by light and electron microscopy. Wistar rats
were instilled through the trachea with papain at a rate of
3 mg/100 g animal weight. The animals were sacrificed
12 h, 3 days, 10 days and 60 days after enzyme
instillation. The "Mean Linear Intercept" (MLI), the
"Number of fenestrations/respiratory units" (NF) the
“Number of macrophages per mm of alveolar wall”
(NM) and the "Number of respiratory unit/mm2” (RU),
both in the control and experimental groups were
studied. Two months after treatment, the experimental
group showed a strong increase in the MLI (p<0.001)
and NF (p<0.001), and a diminished number of RU
(p<0.05) compared with the control group. Partial
correlation analysis showed a positive correlation only
between MLI and NF. Twelve hours after papain
instillation an inflammatory response was observed, the
elastic fibres were ruptured, while the microfibrilar
component remained. New formations of eulanin elastic
fibres were observed three days post papain instillation.
After ten days the interalveolar oedema had disappeared and the elastic fibres were of normal morphology
although irregular groups of strips of elastic fibres were
evident. A mixed pattern of panlobular, centrilobular and
normal lung zones were observed. Two months after
papain instillation abundant accumulations of elastic
fibres of irregular outline were observed associated to
collagen fibres. In conclusion, the morphometric
parameters studied showed a significant progression of
the emphysema. The strong correlation between NF and
MLI suggested that papain-induced emphysema is principally caused by breaches of the alveolar walls. The
results seem to point to a very abnormal remodelling
process associated with elastic fibre regeneration,
although there were no signs of destruction of these new
fibres formed in emphysematous rat lung induced by
papain.
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