Publication: Decreased expression of antioxidant enzymes in the conjunctival epithelium of dry eye (Sjogren s syndrome) and its possible contribution to the development of ocular surface oxidative injuries
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Date
2008
Authors
Cejkova, J. ; Ardan, T. ; Simonová, Z. ; Cejka, C. ; Malec, J. ; Dotrelová, D. ; Brunová, B.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Previous studies have described elevated
lipid peroxidase, myeloperoxidase and xanthine
oxidoreductase/xanthine oxidase levels on the ocular
surface of patients suffering from autoimmune dry eye
(Sjögren’s syndrome, SS). Reactive oxygen species
generated by various enzymatic systems may be
dangerous to the eye if they are not sufficiently cleaved
by antioxidants. Because antioxidants have not been
investigated in dry eye, the aim of this study was to
examine the expression of antioxidant enzymes that
cleave reactive oxygen species and play a key role in
antioxidant protection. Conjunctival epithelial cells of
dry eye (SS) patients were obtained by the method of
impression cytology using Millicell membranes. Normal
eyes served as controls. In the conjunctival epithelium
superoxide dismutase, catalase and glutathione
peroxidase were examined immunohistochemically. The
enzyme expression levels were determined by image
analysis and statistical evaluation. In contrast to normal
eyes, where antioxidant enzymes were highly expressed
in the conjunctival epithelium, in dry eye their
expression was much less pronounced in correlation with the increasing severity of dry eye symptoms. Our
study suggests that the decreased expression of
antioxidant enzymes in dry eye disease (SS) contributes
to the development of anterior eye surface oxidative
injuries.
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