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Leishmania amazonensis isolated from human visceral leishmaniasis: histopathological analysis and parasitological burden in different inbred mice

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dc.contributor.authorFreitas de Souza, Celeste da Silva
dc.contributor.authorCalabrese, Kátia da Silva
dc.contributor.authorAbreu Silva, Ana Lúcia
dc.contributor.authorPereira Carvalho, Luiz Otávio
dc.contributor.authorde Oliveira Cardoso, Flávia
dc.contributor.authorMoraes Cavalheiros Dorval, Maria Elizabeth
dc.contributor.authorTeruya Oshiro, Elisa
dc.contributor.authorQuaresma, Patrícia Flávia
dc.contributor.authorFerreira Gontijo, Célia Maria
dc.contributor.authorda Silva Pacheco, Raquel
dc.contributor.authorDoria Rossi, Maria Isabel
dc.contributor.authorGonçalves da Costa, Sylvio Celso
dc.contributor.authorZaverucha do Valle, Tânia
dc.date.accessioned2022-05-09T10:04:13Z
dc.date.available2022-05-09T10:04:13Z
dc.date.issued2018
dc.description.abstractLeishmania amazonensis is a major etiological agent of human cutaneous leishmaniasis in the Americas; nevertheless there are some reports of this species causing visceral disease in dogs and men. In the present work we have studied a Leishmania strain isolated from a human case of visceral leishmaniasis. We have infected different mouse strains and analyzed the development of the disease, studying the parasite’s ability to visceralize and whether this ability is influenced by host genetics. Female BALB/c, C57BL/6, C57BL/10, CBA, DBA/2, and C3H/He mice were subcutaneously infected with 104 L. amazonensis amastigotes. BALB/c, C57BL/6 and C57BL/10 mice were found to be very susceptible to infection, showing lesions that developed to necrosis and ulceration. CBA mice developed a late but severe lesion. DBA/2 mice developed only discrete lesions, while C3H/He mice did not develop any lesions. All mouse strains except C3H/He showed some degree of visceralization, presenting parasites in the spleen, while BALB/c, C57BL/6 and CBA presented parasites also in the liver. Moreover, most of the strains presented high parasite load at the infection site, whereas DBA and C3H/He mice showed low or no parasite load 90 days after infection, respectively. Histopathology corroborates the results, showing that susceptible mice presented an inflammatory reaction with parasites in the skin, lymph nodes and spleen, while strains that are more resistant presented low parasitism and discrete inflammatory reaction. Results indicate that this isolate is extremely virulent, can easily visceralize and that the pathogenesis of leishmaniasis is, at least in part, related to the genetic background of the host.es
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dc.format.extent12es
dc.identifier.citationHistology and Histopathology, Vol.33, nº7, (2018)
dc.identifier.doiDOI: 10.14670/HH-11-965
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/119722
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectLeishmania amazonensises
dc.subjectVisceralizationes
dc.subjectHistopathological analysises
dc.subjectSusceptibilityes
dc.subjectResistancees
dc.subjectMurine modeles
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleLeishmania amazonensis isolated from human visceral leishmaniasis: histopathological analysis and parasitological burden in different inbred micees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.33, nº7 (2018)