Publication: Selective immunolabeling of early gestational cytotrophoblast and its neoplastic counterpart by the monoclonal antibody Ber- EP4
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Date
1993
Authors
Babury, R.A. ; Moscovic, E.A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
This report presents preliminary observations
on the distribution of an epithelial surface antigen
in human trophoblastic cells studied with the
monoclonal antibody Ber-EP4 by means of an
immunoperoxidase technique. Three cases of gestational
choriocarcinoma and 20 cases of both villous and
extravillous trophoblast, including one complete
hydatidiform mole and one exaggerated placental site,
were examined with a panel of monoclonal antibodies
against epithelial antigen (Ber-EP4), cytokeratin (AE,
and AE3), vimentin (VIM), carcinoembryonic antigen
(CEA) and epithelial membrane antigen (EMA), as well
as with polyclonal antibodies against the beta subunit of
human chorionic gonadotropin (hCG), human placental
lactogen (hPL) and placental alkaline phosphatase
(PIAP). While, as anticipated, al1 currently defined
trophoblastic cell lines expressed low molecular weight
cytokeratin only first trimester cytotrophoblastic cells
and their neoplastic counterpart were found to possess
the epithelial antigen detected by Ber-EP4. This
distinctive property of the cytotrophoblast declined
rapidly after eight weeks of gestational age but persisted
in second trimester molar tissue and was prominently
displayed in choriocarcinoma. By identifying the
presence of cytotrophoblastic cell lines in malignancy
more reliably than can be achieved with conventional
panels of hCG, hPL and PIAP, Ber-EP4 has potential
diagnostic utility in gestational trophoblastic disease.
Our findings also suggest that the monoclonal anti-CEA
antibody may be helpful in distinguishing between
syncytiotrophoblastic cells and non-reactive multinucleate
cells of the intermediate trophoblast.
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