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In vivo toxicity of the ribosome- inactivating lectin ebulin f in elderly mice

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dc.contributor.authorGarrosa, Manuel
dc.contributor.authorJiménez, Pilar
dc.contributor.authorCórdoba Díaz, Damián
dc.contributor.authorGarcía Recio, Verónica
dc.contributor.authorGayoso, Sara
dc.contributor.authorRojo, María Ángeles
dc.contributor.authorGayoso, Manuel J.
dc.contributor.authorGirbés, Tomás
dc.date.accessioned2022-05-19T08:54:53Z
dc.date.available2022-05-19T08:54:53Z
dc.date.issued2018
dc.description.abstractEbulin f is a ribosome-inactivating protein (RIP) present in green fruits of the dwarf elder (Sambucus ebulus L). Since dwarf elder fruits are used for food and as a medicine, we assessed the study of toxicological effects and safety of ebulin f in elderly mice, comparing these results with those reported in young animals and with other RIPs. Female Swiss mice aged 6 and 12 months of age were intraperitoneally injected with a single dose from 1.4 to 4.5 mg/kg ebulin f. Heart, stomach, intestines, lung, kidney, liver, spleen, pancreas, adrenal gland, uterus, ovary and brain were studied. Histology analysis was carried out by staining with hematoxylin and eosin and Masson’s trichrome observed with a light microscope, or apoptosis detection by TUNEL method observed with a confocal laser microscope. Treated animals injected with the lower dose could recover their weights, but after 14 days half of them died. The higher dose caused a progressive loss of body weight leading to death. In the animals of the experimental groups it was found atrophy of Lieberkühn’s crypts, pneumonia, nephronal degeneration, myocardial atrophy, centrolobular hepatic necrosis, splenic white pulp necrosis foci and increased rate of apoptosis in the intestines and liver, in which apoptoses were mainly located in the vicinity of the lobular central vein. We conclude that ebulin f affects vital organs in elderly mice.es
dc.formatapplication/pdfes
dc.format.extent8es
dc.identifier.citationHistology and Histopathology, Vol.33, nº9, (2018)
dc.identifier.doiDOI: 10.14670/HH-11-992
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/120239
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectEbulines
dc.subjectRibosome-inactivating proteines
dc.subjectRIPes
dc.subjectRILes
dc.subjectLectines
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleIn vivo toxicity of the ribosome- inactivating lectin ebulin f in elderly micees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.33, nº9 (2018)