Publication:
Continuous specimen cooling during slicing and thickness measurement contributes to improved accuracy in image analysis of pathologic specimens

Simple item page
dc.contributor.authorSaio, Masanao
dc.contributor.authorKushibiki, Raia
dc.contributor.authorKanehira, Yuki
dc.contributor.authorIshizawa, Akifumi
dc.contributor.authorAbe, Yuka
dc.contributor.authorKobayashi, Sayaka
dc.contributor.authorNishijima, Yoshimi
dc.date.accessioned2025-01-08T10:01:19Z
dc.date.available2025-01-08T10:01:19Z
dc.date.issued2025
dc.description.abstractWe measured section thickness (ST) after slicing using a film thickness meter and investigated the relationship between ST and the percent area of positive staining using computer-assisted image analysis. Methods: Sections were prepared from a paraffin-only block and formalin-fixed paraffin-embedded (FFPE) blocks containing fish sausage and human liver specimens. The ST was compared between the sections prepared with cooling using an ice pack (IP) or a continuous cooling device (CCD) paired with a sliding microtome set at an ST of 4 μm. The sections were stained with eosin or aniline blue, and the association between the percent area of positive staining and ST was determined using computer-aided analysis of images captured with a whole slide scanner. Results: The average STs of the paraffin-only block sections measured by four practitioners were 5.01-5.41 and 4.09-4.33 μm in samples prepared using an IP and a CCD, respectively. Therefore, subsequent analyses included sections prepared using the CCD. The ST of the tissue surface was significantly thinner than that of the paraffin surrounding the tissue section. Furthermore, the percent areas of positive staining for eosin and aniline blue were significantly correlated with ST in both the fish sausage and liver sections. The analysis of the ST and percent area of positive staining in 60 sections of the same block, which were categorized into quantiles based on ST, revealed a significant difference in the percent area of positive staining between the thicker and thinner sections. Discussion: Specimen sectioning should be performed with a CCD, ST should be measured before the staining of pathologic specimens prepared for quantitative analysis, and histologic examination should be performed using specimens with uniform ST.es
dc.formatapplication/pdfes
dc.format.extent8es
dc.identifier.citationHistology and Histopathology Vol. 40, nº01 (2025)
dc.identifier.doihttps://doi.org/10.14670/HH-18-751
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/148021
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relation10.14670/HH-18-751es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectFormalin fixed paraffin embedded blockes
dc.subjectContinuous cooling during slicinges
dc.subjectSlicing accuracyes
dc.subjectSpecimen thickness measurementes
dc.subjectComputer-assisted image analysises
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleContinuous specimen cooling during slicing and thickness measurement contributes to improved accuracy in image analysis of pathologic specimenses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Saio-40-31-38-2025.pdf
Size:
2.54 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.26 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Histology and histopathology Vol.40, nº1 (2025)