Publication:
Brain derived neurotrophic factor maintains Brn3a expression in axotomized rat retinal ganglion cells

Simple item page
dc.contributor.authorSánchez Migallón, María del Cielo
dc.contributor.authorNadal-Nicolás, Francisco Manuel
dc.contributor.authorJiménez López, Manuel
dc.contributor.authorSobrado Calvo, Paloma
dc.contributor.authorVidal Sanz, Manuel
dc.contributor.authorAgudo Barriuso, Marta
dc.contributor.departmentOftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
dc.contributor.otherFacultades de la UMU::Facultad de Medicina
dc.date.accessioned2026-01-27T08:38:21Z
dc.date.available2026-01-27T08:38:21Z
dc.date.copyright © 2011 Elsevier Ltd.
dc.date.issued2009-08-16
dc.description.abstractThe transcription factor Brn3a has been reported to be a good marker for adult rat retinal ganglion cells in control and injured retinas. However, it is still unclear if Brn3a expression declines progressively by the injury itself or otherwise its expression is maintained in retinal ganglion cells that, though being injured, are still alive, as might occur when assessing neuroprotective therapies. Therefore, we have automatically quantified the whole population of surviving Brn3a positive retinal ganglion cells in retinas subjected to intraorbital optic nerve transection and treated with either brain derived neurotrophic factor or vehicle. Brain derived neurotrophic factor is known to delay retinal ganglion cell death after axotomy. Thus, comparison of both groups would inform of the suitability of Brn3a as a retinal ganglion cell marker when testing neuroprotective molecules. As internal control, retinal ganglion cells were, as well, identified in all retinas by retrogradely tracing them with fluorogold. Our data show that at all the analyzed times post-lesion, the numbers of Brn3a positive retinal ganglion cells and of fluorogold positive retinal ganglion cells are significantly higher in the brain derived neurotrophic factor-treated retinas compared to the vehicle-treated ones. Moreover, detailed isodensity maps of the surviving Brn3a positive retinal ganglion cells show that a single injection of brain derived neurotrophic factor protects retinal ganglion cells throughout the entire retina. In conclusion, Brn3a is a reliable retinal ganglion cell marker that can be used to accurately measure the potential effect of a given neuroprotective therapy.
dc.formatapplication/pdf
dc.format.extent8
dc.identifier.citationVision Res. 2009 Nov;49(23):2808-25
dc.identifier.doihttps://doi.org/10.1016/j.exer.2011.02.001
dc.identifier.issn0014-4835
dc.identifier.urihttp://hdl.handle.net/10201/194170
dc.languageeng
dc.publisherElsevier
dc.relationThis work was supported by research grants from Fundación Séneca 04446/GERM/07; Spanish Ministry of Education and Science SAF-22010-10385; Spanish Ministry of Science and Innovation and ISCIII-FEDER: PI07/0225; PI10/00187, PI10/01496 and Red Temática de Investigación Cooperativa en Oftalmología RD07/0062/0001.
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0014483511000352
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectRGC
dc.subjectAxotomy
dc.subjectSurvival
dc.subjectBrn3a
dc.subjectBDNF
dc.subjectSpatial distribution
dc.subject.odsObjetivo 3: Salud
dc.titleBrain derived neurotrophic factor maintains Brn3a expression in axotomized rat retinal ganglion cells
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublicationes
relation.isAuthorOfPublicationba9bf053-48ec-4e39-93ac-ffcbaf10fc0f
relation.isAuthorOfPublicationce26a072-5090-4cfb-b469-5b9a0b5dc462
relation.isAuthorOfPublication8cc42adf-31e9-49c4-a5e2-476346abe217
relation.isAuthorOfPublication5bf27bab-9d43-4b02-a752-5513261676f6
relation.isAuthorOfPublication.latestForDiscoveryba9bf053-48ec-4e39-93ac-ffcbaf10fc0f
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Sánchez-Migallón (ExpEyeRes 2011) Brn3a BDNF Rat.pdf
Size:
1.14 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.37 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos