Publication: Anti-l-light chain-peptide antibodies are suitable for the immunohistochemical classification of AL amyloid
Loading...
Date
2007
Authors
Kuçi, H. ; Ebert, M. ; Röcken, C.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
We aimed to test whether antibodies raised
against recombinant peptides corresponding to the
variable region of immunoglobulin light chains are
suitable for the immunohistochemical classification of
amyloid. The Entrez database of the National Center for
Biotechnology Information (NCBI) was searched for all
protein sequence entries which met the search criteria
“amyloid” and “lambda light chain”. Sixty-four different
l-light chain-derived amyloid protein sequences were
retrieved, aligned and categorized into the V region
subgroups of l-light chain detailed by the NCBI, i.e.
subgroup I (21 protein sequences), II (14), III (6), IV (1),
V (1) and VI (21). V region subgroup I was chosen for
epitope sequence selection and two rabbits were
immunized with the following peptides: NH2-
ISCSGSSSNIGSNTV-CONH2 and NH2-QRPSG
VPDRFSGSKSGTS-CONH2. Sensitivity and specificity
of the IgG-purified antibodies was tested by Western
blotting using amyloid A- (AA), ALl- and ALk-amyloid
proteins, and by immunohistochemistry on tissue
microarrays with 110 different amyloid containing tissue
samples obtained at autopsy from 22 patients, and on 27
biopsy specimens from a series of 24 patients. Our
peptide antibodies specifically stained AL amyloid l-
light chain-origin, in both Western blots and formalinthat peptide-antibodies directed against immunoglobulin-
derived l-light chain proteins can be applied
for the immunohistochemical classification of amyloid.
This offers the opportunity to generate a large set of anti-
l-light chain protein-antibodies for the
immunohistochemical classification of amyloid
independently from native human tissue sources.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.