Publication: Frequent DYRK2 gene amplification in micropapillary
element of lung adenocarcinoma - an implication in
progression in EGFR-mutated lung adenocarcinoma
Authors
Koike, Chihiro ; Okudela, Koji ; Matsumura, Mai ; Mitsui, Hideaki ; Suzuki, Takehisa ; Arai, Hiromasa ; Kataoka, Toshiaki ; Ishikawa, Yoshihiro ; Umeda, Shigeaki ; Tateishi, Yoko ; Ohashi, Kenichi
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-294
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info:eu-repo/semantics/article
Description
Abstract
The present study aimed to discern the
molecular alterations involved in the progression of
EGFR-mutated lung adenocarcinoma (LADC). We
previously demonstrated that the micropapillary (mPAP)
element is the most important histological factor for
assessing malignant grades in LADCs. Therefore, mPAP
and other elements were separately collected from three
cases of EGFR-mutated LADC using laser capture
microdissection and subjected to a comprehensive
mRNA expression analysis. We focused on DYRK2 in
this study because its level showed a substantial increase
in EGFR-mutated LADCs with mPAP. We also
immunohistochemically examined 130 tumors for the
expression of DYRK2. The results confirmed a strong
expression of DYRK2 in EGFR-mutated LADC with
mPAP. Fluorescent in situ hybridization (FISH) analyses
targeting the DYRK2 locus revealed frequent gene
amplification in EGFR-mutated LADC, specifically
occurring in the high-grade components, like mPAP. In
summary, the results of this study suggest that DYRK2
overexpression through gene amplification is one of the
molecular mechanisms responsible for promoting the
progression of EGFR-mutated LADC.
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Citation
Histology and Histopathology Vol. 36, nº3 (2021)
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