Publication: Cryogenic electron microscopy reveals morphologically distinct subtypes of extracellular vesicles among porcine ejaculate fractions
| dc.contributor.author | Parra, Ana | |
| dc.contributor.author | Barranco, Isabel | |
| dc.contributor.author | Martínez Díaz, Pablo | |
| dc.contributor.author | González, Esperanza | |
| dc.contributor.author | Albóniga, Oihane E. | |
| dc.contributor.author | Cabrera, Diana | |
| dc.contributor.author | Falcón Pérez, Juan M. | |
| dc.contributor.author | Roca, Jordi | |
| dc.contributor.department | Medicina y Cirugía Animal | |
| dc.date.accessioned | 2024-09-05T09:48:38Z | |
| dc.date.available | 2024-09-05T09:48:38Z | |
| dc.date.issued | 2024-07-13 | |
| dc.description | ©The Author(s) 2024. This document is the Published version of a Published Work that appeared in final form in Scientific Reports. To access the final edited and published work see https://doi.org/10.1038/s41598-024-67229-w | |
| dc.description.abstract | Seminal plasma (SP) is rich in extracellular vesicles (EVs), which are still poorly studied, especially in livestock species. To better understand their functional role in both spermatozoa and endometrial epithelial cells, proper characterization of EVs is an essential step. The objective was to phenotypically characterize porcine seminal EVs (sEVs) using cryogenic electron microscopy (cryo-EM), which allows visualization of EVs in their native state. Porcine ejaculates are released in fractions, each containing SP from different source. This allows characterization sEVs released from various male reproductive tissues. Two experiments were performed, the first with SP from the entire ejaculate (n:6) and the second with SP from three ejaculate fractions (n:15): the first 10 mL of the sperm-rich ejaculate fraction (SRF-P1) with SP mainly from the epididymis, the remainder of the SRF (SRF-P2) with SP mainly from the prostate, and the post-SRF with SP mainly from the seminal vesicles. The sEVs were isolated by size exclusion chromatography and 1840 cryo-EM sEV images were acquired using a Jeol-JEM-2200FS/CR-EM. The size, electron density, complexity, and peripheral corona layer were measured in each sEV using the ImageJ software. The first experiment showed that sEVs were structurally and morphologically heterogeneous, although most (83.1%) were small (less than 200 nm), rounded, and poorly electrodense, and some have a peripheral coronal layer. There were also larger sEVs (16.9%) that were irregularly shaped, more electrodense, and few with a peripheral coronal layer. The second experiment showed that small sEVs were more common in SRF-P1 and SRF-P2, indicating that they originated mainly from the epididymis and prostate. Large sEVs were more abundant in post-SRF, indicating that they originated mainly from seminal vesicles. Porcine sEVs are structurally and morphologically heterogeneous. This would be explained by the diversity of reproductive organs of origin. | es |
| dc.format | application/pdf | es |
| dc.format.extent | 12 | es |
| dc.identifier.citation | Sci Rep (2024) 14(1):16175 | |
| dc.identifier.doi | https://doi.org/10.1038/s41598-024-67229-w | |
| dc.identifier.issn | Electronic: 2045-2322 | |
| dc.identifier.uri | http://hdl.handle.net/10201/143605 | |
| dc.language | eng | es |
| dc.publisher | Nature Research | |
| dc.relation | The study was funded by grants: PID2020-113493RB-I00/AEI/10.13039/501100011033 funded by the Spanish Ministry of Science and Innovation [MCIN], Madrid, Spain); PID2022-137738NA-I00 funded by MCIN/AEI/10.13039/501100011033/FEDER EU; RYC2021-034546-I funded by MCIN/AEI/10.13039/501100011033 and European Union (NextgenerationEU/PRTR); and 21935/PI/22 funded by the Seneca Foundation (Murcia, Spain). | es |
| dc.relation.publisherversion | https://www.nature.com/articles/s41598-024-67229-w | es |
| dc.rights | info:eu-repo/semantics/openAccess | es |
| dc.rights | Atribución 4.0 Internacional | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
| dc.subject | Cryo-electron microscopy | es |
| dc.subject | Ejaculate fractions | |
| dc.subject | Extracellular vesicles | |
| dc.subject | Seminal plasma | |
| dc.subject | Porcine | |
| dc.title | Cryogenic electron microscopy reveals morphologically distinct subtypes of extracellular vesicles among porcine ejaculate fractions | es |
| dc.type | info:eu-repo/semantics/article | es |
| dspace.entity.type | Publication | es |
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