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TRIB1 facilitates the proliferation and migration of ovarian cancer cells by inducing EMT progression

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dc.contributor
dc.contributor.authorShi Guangyan
dc.contributor.authorHolgersson Kristian
dc.contributor.authorXin Zhen
dc.contributor.authorSzekely Laszlo
dc.contributor.authorDu Qiqiao
dc.contributor.authorJing Xu
dc.contributor.departmentBiología Celular e Histología
dc.date.accessioned2025-10-15T08:54:51Z
dc.date.available2025-10-15T08:54:51Z
dc.date.issued2025
dc.description.abstractAim. Ovarian cancer (OC) is a fatal female malignant tumor that severely impacts the health of women worldwide. Due to the lack of diagnostic biomarkers, 70% of OC patients are considered in the advanced stage at the first diagnosis. Exploring novel biomarkers for OC diagnosis has become an urgent clinical need to address. TRIB1 is a newly discovered oncogene in several malignant tumors, including acute myeloid leukemia, prostate cancer, and breast cancer. However, the biological function of TRIB1 in OC remains uncertain and, therefore, was explored in the present study. Methods. Levels of TRIB1 in OC and normal tissues were evaluated in the GEPIA database. TRIB1-KD was constructed in ES-2 cells and TRIB1-OE was constructed in OVCAR3 cells using a siRNA and OE vector, respectively. The proliferation ability was determined using the CCK-8 and clone formation assays. The migration ability was detected using the wound healing and Transwell assays. The expression of epithelial-mesenchymal transition (EMT) biomarkers was determined using western blotting. Results. TRIB1 was markedly upregulated in OC tissues compared with normal ovarian tissues in the GEPIA database. The TRIB1 level was slightly altered among ES-2, CAOV3, and SKOV3 cells, with the highest expression in ES-2 cells, which was greatly reduced in OVCAR3 cells. In TRIB1-KD ES-2 cells, a remarkably reduced proliferation ability was observed with the CCK-8 and clone formation assays, accompanied by a reduction in migration distance in the Wound healing assay and the number of migrated cells in the Transwell assay. In contrast, in TRIB1-OE OVCAR3 cells, increased proliferation ability was observed, accompanied by increased migration distance and number of migrated cells. Furthermore, EMT progression was markedly repressed in TRIB1-KD ES-2 cells and remarkably enhanced in TRIB1-OE OVCAR3 cells. Conclusion. TRIB1 facilitated the proliferation and migration of OC cells by enhancing EMT progression
dc.formatapplication/pdf
dc.format.extent9
dc.identifier.citationHistology and Histopathology, Volúmen 40, nº 9 (2025)
dc.identifier.doihttps://doi.org/10.14670/HH-18-868
dc.identifier.eissn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/166569
dc.languageeng
dc.publisherUniversidad de Murcia, Departamento de Histología e Histopatología
dc.relationSin financiación externa a la Universidad
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectOvarian cancer
dc.subjectTRIB1
dc.subjectProliferation
dc.subjectEpithelial-mesenchymal transition
dc.subjectMigration
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleTRIB1 facilitates the proliferation and migration of ovarian cancer cells by inducing EMT progression
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
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Histology and histopathology Vol.40, nº9 (2025)