Publication: Electron microscopic study of the effects of endotoxin on the cells of the hepatic sinusoid in normal and BCG sensitized mice
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Date
1991
Authors
McCuskey, Patricia A. ; McCuskey, Robert S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Electron microscopic studies were conducted
to access ultrastructural alterations in Kupffer cells and
other cells lining the hepatic sinusoids at the peak of
mediator release two hours after challenge with low
doses of endotoxin under various conditions including
reticuloendothelial system (RES) expansion and
activation with BCG. BCG is known to sensitize animals
to endotoxin rendering normally innocuous, low doses of
endotoxin lethal.
Low non-lethal doses (5 pg) of endotoxin activated
Kupffer cells as well as caused isolated foci of cellular
injury. However, animals which were treated with BCG
had a highly activated and expanded RES system as
evidenced by enlarged Kupffer cells with many extended
cellular processes. Granulomas were prevalent and
many reactive cells were present. After two hours
marked cellular injury occurred to sinusoid lining and
parenchymal cells when BCG treated animals were
challenged with these same low doses of endotoxin.
Cellular debris, fibrin, and platelets were observed in
sinusoids often associated with Kupffer cells. These
results suggest that the functional state of Kupffer cells
is an important determinant in the host response to
endotoxin. While there appears to be an effective
clearance of endotoxin; the release of mediators by the
highly activated Kupffer cells can be toxic causing
hepatocellular injury.
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