Publication:
Topotecan hydrochloride effects on retinal vessels in newborn rats

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dc.contributor.authorPescosolido, Nicola
dc.contributor.authorPascarella, Antonia
dc.contributor.authorScarsella, Gianfranco
dc.contributor.authorTafani, Marco
dc.contributor.authorStefanini, Stefania
dc.contributor.authorNebbioso, Marcella
dc.date.accessioned2017-03-10T18:58:52Z
dc.date.available2017-03-10T18:58:52Z
dc.date.issued2012
dc.description.abstractA physiological system, i.e. rodent retina during vessel formation and hierarchical organization, was utilised for assaying antiangiogenic properties of Topotecan, a topoisomerase I inhibitor, capable of inhibiting tumoral growth in animal models of retinoblastoma. In particular we analysed possible differences in effectiveness and side effects among different drug dosages and ways of administration. In the present research only qualitative analyses were undertaken. After preliminary experiments, in which suckling animals subcutaneously treated with Topotecan dosages comprised between 9 and 3 mg/kg underwent high lethality and extremely severe systemic damages, 7 day-old rats were subcutaneously, intravenously or peribulbary injected with a single dose of 1 mg/kg; retinal vessels were visualized in retinal fluorangio-graphies taken 1 and 2 weeks after treatment. The most important and frequent alterations were found to affect radial vessels, which showed non-perfused and/or regionally mislocated segments, together with abnormal branching and enlargements in retinal periphery; persistence of capillary-free periarteriolar regions, non-vascularised regions and spots of extravascular FITC were also detected. Despite the high individual variability the alterations were substantially similar among the different ways of drug administration, while they appeared milder in 21 day-old rats, with respect to younger ones. The extensive vascular remodelling found after Topotecan administration, besides demonstrating the antiangiogenic properties of this chemioterapic drug, confirms the rodent retina as a highly valuable model system for studying angiogenesis modulationes
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.citationHistology and histopathology, Vol. 27, nº 4 (2012)
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/52426
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectAngiogenesises
dc.subjectPeribulbar injectiones
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleTopotecan hydrochloride effects on retinal vessels in newborn ratses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.27, nº 4 (2012)