Publication: Horse adipose-derived mesenchymal
stromal cells constitutively produce
membrane vesicles: a morphological study
Authors
Pascucci, L. ; Dall’Aglio, C. ; Bazzucchi, C. ; Mercati, F. ; Mancini, M.G. ; Pessina, A. ; Alessandri, G. ; Giammarioli, M. ; Dante, S. ; Brunati, G. ; Ceccarelli, P.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
https://doi.org/10.14670/HH-30.549
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info:eu-repo/semantics/article
Description
Abstract
Mesenchymal stromal cells (MSCs) are
multipotent somatic cells that can differentiate into a
variety of mature cell types. Over recent years, their
biological in vitro and in vivo properties have elicited
great expectations in the field of regenerative medicine,
immunotherapy and tumour treatment. An increasing
number of experimental observations suggest that their
biological effects are probably related to a paracrine
mechanism via the release of trophic factors and
cytokines as well as through the production of
membrane vesicles (MVs). These are nanometric
membrane-bound structures, comprising shedding
vesicles (SV) and exosomes (Ex), that enclose and
transfer signalling molecules to target cells.
We hypothesized that MVs may be implicated in the
biological effects of MSCs from horse adipose tissue (EAdMSCs), a type of MSC that has been extensively
studied in recent years for its remarkable efficacy in
tissue regeneration.
By means of electron microscopy, we ascertained,
for the first time, that equine adipose-derived MSCs
constitutively produce MVs (E-AdMSCs). The analysis
of MVs separated by ultracentrifugation allowed us to
describe their general morphological features. Through
the examination of cell monolayers by TEM,
additionally, we distinguished the different pathways of
SV and Ex formation, demonstrating that both fractions
are produced by E-AdMSC.
The accurate description of MV heterogeneous
morphological characteristics led us to emphasize the
possible implications of the relationship between
different morphologies versus different functions.
The data presented in this paper has an additional
value, as they can be noteworthy for horses as well as for
other mammalian species, including humans.
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Citation
Histology and Histopathology, Vol. 30, n.º 5 (2015)
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