Roles of selenoprotein antioxidant protection in zebrafish, Danio rerio, subjected to dietary oxidative stress

Betancor, M B; Almaida Pagán, Pedro Francisco; Spreague, M; Hernandez, A; Tocher, D R

Publication:
Roles of selenoprotein antioxidant protection in zebrafish, Danio rerio, subjected to dietary oxidative stress

dc.contributor.author	Betancor, M B
dc.contributor.author	Almaida Pagán, Pedro Francisco
dc.contributor.author	Spreague, M
dc.contributor.author	Hernandez, A
dc.contributor.author	Tocher, D R
dc.contributor.department	Fisiología
dc.date.accessioned	2024-06-18T11:21:57Z
dc.date.available	2024-06-18T11:21:57Z
dc.date.issued	2015-03-07
dc.description	© Springer Science+Business Media Dordrecht 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Fish Physiology and Biochemistry. To access the final edited and published work see https://doi.org/10.1007/s10695-015-0040-2
dc.description.abstract	In vertebrates, selenium (Se) is an essential micronutrient for vertebrates that is involved in antioxidant protection and thyroid hormone regulation among other roles and functions through its incorporation into proteins, the selenoproteins. Long-chain polyunsaturated fatty acids, such as docosahexaenoic acid (DHA), are essential nutrients for fish although high dietary levels may lead to increased oxidative stress due to the high degree of unsaturation. The present study investigated the effects of Se supplementation on zebrafish, Danio rerio, oxidative status together with selenoprotein expression profiles when subjected to a high-DHA diet. Fish were fed for 8 weeks with one of the four experimental diets, containing high or low-DHA in combination with or without organic Se (7 mg/kg). Fish performance, Se content, fatty acid composition and TBARS of zebrafish were determined, as well as gene expression of selected selenoproteins in liver and muscle. The Se levels in whole fish reflected dietary content. High dietary DHA increased oxidative stress as indicated by reduced growth and high TBARS content, although Se supplementation reduced oxidation. The expression patterns of selenoproteins varied between liver and muscle with only deiodinase type II displaying a transcriptional response when high dietary Se was supplied. High dietary DHA decreased selenoprotein expression in muscle and sps2 expression in liver regardless of the dietary Se content. These data suggest that oxidative stress protection associated with a high dietary intake of Se may not be solely mediated by transcriptional changes in teleost selenoprotein expression.	es
dc.format	application/pdf	es
dc.format.extent	16	es
dc.identifier.citation	Fish Physiology and Biochemistry, 2015, Volume 41, pp 705–720
dc.identifier.doi	https://doi.org/10.1007/s10695-015-0040-2
dc.identifier.issn	Print: 0920-1742
dc.identifier.issn	Electronic: 1573-5168
dc.identifier.uri	http://hdl.handle.net/10201/142391
dc.language	eng	es
dc.publisher	Springer	es
dc.relation	This research was funded by a Marie Curie Intra-European Fellowship within the 7th Community Framework Programme (PIEF-GA-2011-297964, OLDMITO) awarded to P.F.A.-P.	es
dc.relation.publisherversion	https://link.springer.com/article/10.1007/s10695-015-0040-2	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Zebrafish	es
dc.subject	Selenium
dc.subject	Docosahexaenoic acid
dc.subject	Oxidative stress
dc.subject	Selenoproteins
dc.title	Roles of selenoprotein antioxidant protection in zebrafish, Danio rerio, subjected to dietary oxidative stress	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es