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Long-term haematological analysis in pigs derived from assisted reproductive technologies

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dc.contributor.authorMikita, Małgorzata
dc.contributor.authorCánovas, Sebastián
dc.contributor.authorHeras, Sonia
dc.contributor.authorRamis, Guillermo
dc.contributor.authorGadea, Joaquín
dc.contributor.authorCoy, Pilar
dc.contributor.authorRomar, Raquel
dc.contributor.authorRomero Aguirregomezcorta, Jon
dc.contributor.departmentAnatomía y Anatomía Patológica Comparadas
dc.date.accessioned2025-09-04T06:55:11Z
dc.date.available2025-09-04T06:55:11Z
dc.date.copyright© 2025, los autores.
dc.date.issued2025
dc.description.abstractRising livestock demand has increased use of assisted reproductive technologies (ART). Evidence indicates long-term effects of ART on molecular physiology and metabolism. This study explores ART’s impact on haematological parameters in a colony of pigs from 1 to 5 years of age. Animals were born after artificial insemination (AI) and transfer of embryos in vitro produced with (RF-IVP) or without (C-IVP) oviductal and uterine fluids during in vitro fertilization and embryo culture media (París-Oller et al., J Anim Sci and Biotechnol, 2021). Pigs were maintained under identical conditions and sampled every six months. Number of animals in each group decreased throughout life: 33 to 9 (AI); 22 to 10 (C-IVP); and 14 to 4 (RF-IVP). The number of blood samples was the same as the number of animals and were collected via jugular venipuncture in lithium heparin tubes and immediately transported to laboratory. A haematology analyzer (Siemens ADVIA® 120, USA) assessed red blood cell (RBC) count, haemoglobin concentration (HB), haematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), cell haemoglobin concentration mean (CHCM), red blood cell distribution width (RDW), haemoglobin concentration distribution width (HDW), white blood cell (WBC) count; and the differential count of neutrophils, lymphocytes, monocytes, eosinophils, and basophils. Reticulocyte percentage, mean corpuscular volume of reticulocytes (MCVr), haemoglobin content in reticulocytes (CHr) and platelet count were analyzed. Data were analyzed by mixed-effects model and likelihood-ratio test to determine effects of age, group and sex on the variables. Post-hoc test for multiple comparisons Tukey’s method were used. P<0.05 was considered significant. Age affected all variables. MCH, CH, CHCM, WBC, and platelet indices (MPV, MPC, MPM, PMDW) increased through life. Biphasic trajectories occurred in MCV, HTC, RBC, neutrophil counts, monocyte counts, PLT counts, and lymphocytes (secondyear decline followed by increase). Progressive decline with age was observed in monocyte percentage and reticulocyte parameters. Irregular oscillatory patterns dominated HB, RDW, MCHC, CHDW, HDW, eosinophil and basophil parameters, and advanced platelet indices (large PLT, PCT, PDW, MCVr, PCDW). HCT, RBC, and HB reached lowest values in AI group, highest in RF-IVP. HCM, MCHC, and lymphocyte percentage showed lowest means in AI, highest in C-IVP. RDW, WBC, neutrophil parameters, and lymphocyte count were lowest in C-IVP, highest in AI. Monocyte count exhibited lowest values in C-IVP, highest in RF-IVP group. CH and PCDW were higher in females than males, whereas HDW, PDW, MPC were higher in males. Long-term results confirm slight persisting haematological differences in naturally and artificially conceived pigs, although the clinical relevance is unnoticeable.es
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dc.format.extent1
dc.identifier.citationAnim Reprod. 2025 22(3)
dc.identifier.urihttp://hdl.handle.net/10201/158138
dc.languageenges
dc.publisherBrazilian College of Animal Reproduction
dc.relationThis study is part of project PID2020-113366RB-I00 funded by MCIN/AEI/10.13039/501100011033/ and “FEDER Una manera de hacer Europa”.es
dc.relation.ispartof41st Annual Meeting of the Association of Embryo Technology in Europe (AETE)es
dc.relation.publisherversionhttps://animal-reproduction.org/current#nav3es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPorcinees
dc.subjectMetabolismes
dc.subjectARTes
dc.titleLong-term haematological analysis in pigs derived from assisted reproductive technologieses
dc.typeinfo:eu-repo/semantics/lecturees
dspace.entity.typePublicationes
relation.isAuthorOfPublication3cc83bb3-8c44-471d-bc14-7d7dff677509
relation.isAuthorOfPublication.latestForDiscovery3cc83bb3-8c44-471d-bc14-7d7dff677509
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