Publication: The effect of thymic inoculation to induce tolerance of allogeneic thyroid grafts in the outbred rabbit
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Date
1998
Authors
Torchia, M.G. ; Aitken, R.M. ; Thliveris, A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Many studies have demonstrated that
allograft tolerance can be achieved in inbred rats and
mice following intrathymic injection of donor cells or
antigen and treatment with antilymphocyte serum
(ALS). In outbred dogs, xenografts, and inbred rat
strains with major MHC antigen difference, tolerance
has not similarly been induced. The focus of this study
was to determine whether allogeneic thyroid graft
tolerance could be achieved in outbred rabbits. In the
experimental group (n=5), recipients received an
intrathymic injection of donor lymphocytes and a single
treatment of ALS. Controls (n=5) received intrathymic
cell culture medium and ALS treatment. Donor-recipient
allogenicity was monitored with mixed lymphocyte
culture (MLC) over 18 weeks. Donor thyroid tissue was
placed into recipient gluteal muscle fibres one week
following the last MLC measurement. A third group of
rabbits (n=4) received thyroid autografts without any
other treatment. There were no differences in MLC
stimulation indices (SI) between the control and
experimental group nor did MLC (SI) change within
groups. All thyroid autografts survived the two week
monitoring period and demonstrated normal appearing
thyroid follicles on histologic examination. All thyroid
allografts showed severe acute rejection reactions on
biopsy within one week. Further studies using outbred
animals to examine the role of thymic inoculation are
required to determine whether similar techniques might
be successful in the human.
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