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Increased glucosinolate production in Brassica oleracea var. italica cell cultures due to coronatine activated genes involved in glucosinolate biosynthesis

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dc.contributor.authorSánchez Pujante, P.J.
dc.contributor.authorAlmagro Romero, Lorena
dc.contributor.authorBelchí Navarro, Sarai
dc.contributor.authorPedreño García, María Ángeles
dc.contributor.authorSabater Jara, Ana Belén
dc.contributor.departmentBiología Vegetal
dc.date.accessioned2025-05-16T12:05:49Z
dc.date.available2025-05-16T12:05:49Z
dc.date.issued2018-12-19
dc.description© 2018 American Chemical Society. This document is the Published Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry. To access the final edited and published work see https://doi.org/10.1021/acs.jafc.8b04298
dc.description.abstractIn this work, the effect of different elicitors and culture conditions on the production of glucosinolates in broccoli cell cultures was studied. The results showed that 0.5 μM coronatine was the best elicitor for increasing glucosinolate production (205-fold increase over untreated cells after 72 h of treatment). Furthermore, the expression levels of some genes related to the biosynthetic pathway of glucosinolates as well as three Myb transcription factors also have been studied. The highest glucosinolate levels found in coronatine-treated cells were closely correlated with the highest gene expression levels of Cyp79b2, Cyp83b1, St5a, Myb51, and Myb122 after 6 h of treatment. The data shown in this study provide new insight into the key metabolic steps involved in the biosynthesis of glucosinolates, which will be of use for future applications of metabolic engineering techniques in broccoli.
dc.embargo.terms1-ene-2999
dc.formatapplication/pdfes
dc.format.extent10
dc.identifier.citationJournal of Agricultural and Food Chemistry, 2019, Vol. 67, Issue 1, pp. 102–111
dc.identifier.doihttps://doi.org/10.1021/acs.jafc.8b04298
dc.identifier.issnPrint: 0021-8561
dc.identifier.issnElectronic: 1520-5118
dc.identifier.urihttp://hdl.handle.net/10201/154787
dc.languageenges
dc.publisherAmerican Chemical Society
dc.relationMinisterio de Economía y Competitividad (no. BIO2017-82374-R), Fundación Seneca-Agencia de Ciencia y Tecnología de la Región de Murcia (no. 19876/GERM/15).es
dc.relation.publisherversionhttps://pubs.acs.org/doi/10.1021/acs.jafc.8b04298
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectBroccoli suspension cultured cells
dc.subjectCoronatine
dc.subjectGene expression
dc.subjectGlucosinolate
dc.titleIncreased glucosinolate production in Brassica oleracea var. italica cell cultures due to coronatine activated genes involved in glucosinolate biosynthesises
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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