Publication: Ex vivo and in vivo modulatory effects of umbilical cord Wharton’s jelly stem cells on human oral mucosa stroma substitutes
Authors
Alfonso-Rodríguez, C.A. ; González-Andrades, E. ; Jaimes-Parra, B.D. ; Fernández-Valadé, R. s ; Campos, A. ; Sánchez-Quevedo, M. C. ; Alaminos, M. ; Garzon, I.
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
10.14670/HH-11-628
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info:eu-repo/semantics/article
Description
Abstract
Novel oral mucosa substitutes have been
developed in the laboratory using human umbilical cord
Wharton’s jelly stem cells -HWJSC- as an alternative
cell source. In the present work, we have generated
human oral mucosa substitutes with oral mucosa
keratinocytes and HWJSC to determine the influence of
these cell sources on stromal differentiation. First,
acellular and cellular stroma substitutes and bilayered
oral mucosa substitutes with an epithelial layer
consisting of oral mucosa keratinocytes -OM samplesor HWJSC -hOM- were generated. Then, tissues were
analyzed by light and electron microscopy, histochemistry and immunohistochemistry to quantify all
major extracellular matrix components after 1, 2 and 3
weeks of ex vivo development, and OM and hOM were
also analyzed after in vivo grafting. The results showed
that bioengineered oral mucosa stromas displayed an
adequate fibrillar mesh. Synthesis of abundant collagen
fibers was detected in OM and hOM after 3 weeks, and
in vivo grafting resulted in an increased collagen
synthesis. No elastic or reticular fibers were found.
Glycoprotein synthesis was found at the epithelialstromal layer when samples were grafted in vivo. Finally,
proteoglycans, decorin, versican and aggrecan were
strongly dependent on the in vivo environment and the
presence of a well-structured epithelium on top. The use
of HWJSC was associated to an increased synthesis of
versican. These results confirm the usefulness of fibrinagarose biomaterials for the generation of an efficient
human oral mucosa stroma substitute and the importance
of the in vivo environment and the epithelialmesenchymal interaction for the adequate differentiation
of the bioengineered stroma.
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Citation
Histology and Histopathology, vol.30, nº 11, (2015)
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