Publication: The activity of paraoxonase type 1 (PON-1) in boar seminal plasma and its relationship with sperm quality, functionality, and in vivo fertility
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Date
2015-01-19
Authors
Tvarijonaviciute, Asta ; Pérez-Patiño, Cristina ; Alkmin, Diego V. ; Cerón, José J. ; Martínez, Emilio A. ; Rodríguez-Martínez, Heriberto ; Roca, Jordi ; Barranco Cascales, Isabel
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Publisher
Wiley
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DOI
https://doi.org/10.1111/andr.309
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info:eu-repo/semantics/article
Description
© 2015 American Society of Andrology and European Academy of Andrology. This document is the Published version of a Published Work that appeared in final form in Andrology. To access the final edited and published work see https://doi.org/10.1111/andr.309
Abstract
Paraoxonase 1 (PON-1) is a hydrolytic enzyme present in body fluids, capable of protecting cells against oxidative stress. The hypothesis was hereby to test that PON-1, present in seminal plasma (SP), acts protecting boar spermatozoa when showing a reasonable high activity in the ejaculate. SP-PON-1 activity differed (p < 0.001) among boars (from 0.10 to 0.29 IU/mL). Intra-boar variability was also observed (p < 0.05), but only in two of the 15 boars. SP-PON-1 activity differed among ejaculate portions, showing the spermatozoa-peak portion of spermatozoa-rich ejaculate fraction the highest levels (0.35 ± 0.03 IU/mL, ranging from 0.12 to 0.69) and the post-sperm ejaculate fraction the lowest levels (0.12 ± 0.01 IU/mL, ranging from 0.03 to 0.21). SP-PON-1 activity was positively correlated with the percentage of spermatozoa with rapid and progressive movement (p < 0.01) and negatively correlated with the generation of intracellular reactive oxygen species (p < 0.01) in semen samples after 72 h of liquid storage. SP-PON-1 activity was highest (p < 0.01) in boars with highest farrowing rates. In conclusion, SP-PON-1 activity differed among boars and ejaculate fractions/portions. SP-PON-1 activity was positively correlated with sperm quality and functionality of liquid-stored semen samples and it evidenced a positive association with in vivo fertility.
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Citation
Andrology, 2015, Vol. 3, N. 2, pp. 315-320.
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1-ene-2999
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