Publication: Vestibular histofluorescence could be due to accumulation of both the antibiotic and its derivative, streptidine, after
acute streptomycin treatment in the guinea pig
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Date
2001
Authors
Meza Ruiz, G. ; Barba-Behrens, N. ; Granados, O. ; Hernandez-Cruz, A. ; Toxqui, A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Acute treatment with 300 mglkg of
pigmented guinea pigs with streptomycin sulfate induces
an elevation of endogenous fluorescence in vestibular
ampullary cristae. Fluorescence accumulates in all
compartments of the epithelium, i.e., vestibular sensory
and supporting cells and nerve fibers of the stroma and it
was very intense 1 and 12 hours after its administration.
Fli~orescence decreased to control levels 24 hours
following streptomycin injection. Fluorescence levels
were very low either in untreated animals or in animals
injected with s a l i n e physiological solution. To
investigate whether this fluorescence was an intrinsic
property of the antibiotic or whether it was due to a
derivative of it, or both, an in vitro fluorescence
spectrum was performed with I00 ,LIM solutions of
streptomycin or streptidine, or both, dissolved in various
buffer solutions at 488 nm of excitation.
A discrete level of fluorescence was observed in the
spectrum regardless of media when separate solutions of
both streptomycin or streptidine were s t u d i e d .
Fluorescence notably increased at 522-532 nm when the
solutions contained both streptomycin and streptidine
toget her.
These results suggest that streptidine putatively derived from streptomycin may contribute to the
observed fluorescence accumulation in vestibular
preparations after acute treatment. Thus, these metabolic
properties of the inner ear which transform streptomycin
into streptidine, something never considered earlier,
could be claimed as partially responsible for converting
a therapeutic agent into a compound which could be as
harmful as STP to the inner ear.
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