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Biocompatibility of three new calcium silicate-based endodontic sealers on human periodontal ligament stem cells

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dc.contributor.authorCollado-González, Mar
dc.contributor.authorGarcía Bernal, David
dc.contributor.authorOñate Sánchez, Ricardo Elías
dc.contributor.authorOrtolani-Seltenerich, P.S.
dc.contributor.authorLozano, Adrián
dc.contributor.authorForner, Leopoldo
dc.contributor.authorLlena, Carmen
dc.contributor.authorRodríguez Lozano, Francisco Javier
dc.contributor.departmentDermatología, Estomatología, Radiología y Medicina Física
dc.date.accessioned2025-09-25T07:56:02Z
dc.date.available2025-09-25T07:56:02Z
dc.date.copyright© 2016 International Endodontic Journal. Published by John Wiley & Sons Ltd
dc.date.issued2016-09-26
dc.description.abstractAim To evaluate the biocompatibility of three calcium silicate-based endodontic sealers, Bioroot BC Sealer (Septodont, Saint-Maur-des-Fosses, France), Endoseal MTA (EndoSeal, Maruchi, Seoul, Korea) and Nano-ceramic Sealer (B&L Biotech, Fairfax, VA, USA) (NCS), on human periodontal ligament stem cells (hPDLSCs). Methodology human periodontal ligament stem cells were cultured in the presence of various endodontic sealer eluates for 24 h. Cell viability was determined using the MTT assay. Cell death and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. Also, an in vitro scratch wound-healing model was used to determine their effects in cell migration. Finally, to assess cell morphology and attachment to the different sealers, hPDLSCs were directly seeded onto the material surfaces and analysed by scanning electron microscopy (SEM). One-way analysis of variance (ANOVA) followed by a Bonferroni post-test was performed (P < 0.05). Results At 24 h, cell spreading was evident in the presence of Bioroot BC Sealer (BR) and Nano-ceramic Sealer (NCS), but not Endoseal MTA (ES). At 72 h, BR and NCS exhibited high and moderate cell proliferation, respectively, whereas ES revealed low rates of cell proliferation (P < 0.05). Similar results were obtained in a cell death assay. In addition, hPDLSCs maintained their mesenchymal phenotype in all conditions although their capacity to migrate was higher in the presence of BR. Finally, SEM studies revealed a good degree of proliferation, cell spreading and attachment, especially when using BR and NCS discs. Conclusions BR and NCS were associated with better cytocompatibility than ES. Further in vitro and in vivo investigations are required to confirm the suitability of these calcium silicate-based endodontic sealers for clinical application.
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dc.format.extent10
dc.identifier.citationInternational Endodontic Journal, 2017, 50(9):875-884
dc.identifier.doihttps://doi.org/10.1111/iej.12703
dc.identifier.eissn1365-2591
dc.identifier.issn0143-2885
dc.identifier.urihttp://hdl.handle.net/10201/161549
dc.languageeng
dc.publisherWilley
dc.relationThis work was supported by the Spanish Net of Cell Therapy (TerCel) provided by the Carlos III Institute of Health (ISCiii). The authors deny any conflict of interests related to this study
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1111/iej.12703
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleBiocompatibility of three new calcium silicate-based endodontic sealers on human periodontal ligament stem cells
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
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relation.isAuthorOfPublication2372b160-a380-45dd-835a-6f377fbeaf97
relation.isAuthorOfPublicationdd1715f8-fe64-4ed6-8cf2-dc327b3020e6
relation.isAuthorOfPublication32059c24-bbdc-4ba3-a281-72ad733fc960
relation.isAuthorOfPublication.latestForDiscoveryba32f760-d55c-40a7-9452-62dfd47cbeaf
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