Publication: Lectin binding patterns in normal canine endometrium and in bitches with
pyometra and cystic endometrial hyperplasia
Authors
Leitner, M. ; Aurich, J.E. ; Galabova, G. ; Aurich, C. ; Walter, I.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Cystic endometrial hyperplasia (CEH) and
pyometra in the bitch are dioestral syndromes, supposed
to be caused by hormonal disturbances and changes in
endometrial steroid hormone receptor levels.
Histologically, the endometria show cystic dilated glands
and, if bacteria succeed in invading the uterus, pyometra
may develop in the following metoestrus. In this study,
lectin histochemistry was performed on paraffin sections
to compare carbohydrate expression of uterine glands
and surface epithelium in healthy dogs and in dogs with
CEH and pyometra. Lectin binding is a useful tool to
identify glycoconjugates, especially of the glycocalyx,
which has essential functions in the endometrium during
reproduction. Uterine tissue was obtained from 18
healthy bitches in metoestrus or anoestrus and 18 bitches
with a clinical diagnosis of CEH or pyometra. Normal
endometria showed cycle-dependent changes in SBA,
PNA, HPA and UEA binding during metoestrus and
anoestrus. LCA did not show cycle-dependent changes
and WGA bound to Golgi regions in the apical parts of
surface epithelial cells only in metoestrous. Endometria
with inflammatory alterations lost cycle-specific lectin
binding patterns and, with increasing severity of
pathological changes, showed a marked decrease in binding intensity to the glandular and surface epithelial
glycocalyx and secretions. In dogs with CEH, unaltered
glands with generally strong lectin binding to the
glycocoalyx and Golgi regions were found adjacent to
altered glands. The decrease of lectin binding in
pyometra cases is supposed to be a result of glandular
exhaustion after cystic hyperplasia. In addition, bacterial
adhesion to sugar residues on the uterine surface
epithelium might impede lectin binding.
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