Publication: Myenteric plexus of obese diabetic mice (an animal model of human type 2 diabetes)
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Date
2001
Authors
Spangeus, A. ; El-Salhy, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The myenteric plexus of the gastrointestinal
tract was investigated in the obese diabetic mouse, an
animal model of human type 2 diabetes. Sections were
immunostained by the avidin-biotin complex method,
using a general nerve marker, protein gene product 9.5
(PGP 9.5), as well as antibodies to several important
neurotransmitters. Computerized image analysis was
used for quantification. When diabetic mice were
compared with controls, no difference was found in the
density of PGP 9.5-immunoreactive (IR) nerve fibres in
antrum, duodenum or colon. In antrum and duodenum,
diabetic mice showed a decreased number of vasoactive
intestinal peptide (V1P)-IR neurons in myenteric ganglia
as well a decreased relative volume density in myenteric
plexus (though not significantly in antrum, p=0.073). No
difference was found regarding VIP-IR nerves in colon.
The volume density of nitric oxide synthase (NOS)-IR
nerve fibres was decreased in antrum and duodenum of
diabetic mice, whereas no difference was found in colon.
The density of galanin-IR neme fibres was decreased in
duodenum. Whereas neuropeptide Y (NPY)- and
vesicular acetylcholine transporter (VAChT)-IR nerve
fibres was increased in density in colon of diabetic mice, no difference was found in antrum and duodenum.
Regarding substance P, there was no difference between
diabetic and control mice in antrum, duodenum or colon.
The present study shows that gut innervation is affected
in this animal model of human type 2 diabetes. It is
possible that the present findings may have some
relevance for the gastrointestinal dysfunctions seen in
patients with type 2 diabetes.
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