In vitro effects of a natural marine algae polysaccharide (λ-carrageenin) on seabream erythrocytes, tumour cell lines and marine bacterial pathogens

Campos-Sánchez, José Carlos; Esteban Abad, María de los Ángeles; Guardiola Abellán, Francisco Antonio

Publication:
In vitro effects of a natural marine algae polysaccharide (λ-carrageenin) on seabream erythrocytes, tumour cell lines and marine bacterial pathogens

dc.contributor.author	Campos-Sánchez, José Carlos
dc.contributor.author	Esteban Abad, María de los Ángeles
dc.contributor.author	Guardiola Abellán, Francisco Antonio
dc.contributor.department	Biología Celular e Histología
dc.date.accessioned	2023-05-08T10:52:05Z
dc.date.available	2023-05-08T10:52:05Z
dc.date.created	2023-05-08
dc.description.abstract	Carrageenin is a high molecular weight sulphated mucopolysaccharide used to induce experimental inflammation in mammals. In addition, it possesses a wide variety of properties that have not yet been studied in fish. This study evaluated the hemagglutinating, hemolytic, cytotoxic, and antibacterial activities of λ-carrageenin. The results showed that λ-carrageenin has hemagglutinating and hemolytic activities on gilthead seabream erythrocytes, which were dose and time-dependent during the first 6 hours of incubation. No significant effects on the haemolytic activity of erythrocytes were observed after incubation for 12 or 24 h with λ-carrageenin. The PLHC-1 cell line showed significant increases in cytotoxic activity after 6 or 12 hours of incubation compared with control cells, and the highest doses of λ-carrageenin caused cytotoxicity in PLHC-1 cells after 24 h of incubation. The morphology of PLHC1 cells incubated with the highest doses of λ-carrageenin for 12 or 24 h showed obvious cell death changes compared with control cells. Interestingly, no significant variations in cytotoxic activity were observed in SAF-1 or HeLa cell lines after incubation with λ-carrageenin. λ-carrageenin showed significant dose-dependent bactericidal activity against Photobacterium damselae but had no significant effect on the bactericidal activity of Vibrio harveyi, V. anguillarum, and Tenacibaculum maritimum. The study suggests that λ-carrageenin has potential applications in aquaculture and aquatic pharmaceutical industries as a hemagglutinating, hemolytic, and antibacterial agent.	es
dc.format	application/vnd.ms-excel	es
dc.format.extent	1	es
dc.identifier.eisbn	Fish and Shellfish Immunology, 2023	es
dc.identifier.uri	http://hdl.handle.net/10201/130769
dc.language	eng	es
dc.relation	This research forms part of the ThinkInAzul programme supported by MCIN with funding from European Union Next Generation EU (PRTR-C17. I1) and by the Comunidad Autónoma de la Región de Murcia-Fundación Séneca (Spain).	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	λ-carrageenin	es
dc.subject	Erythrocytes	es
dc.subject	Fish cell lines	es
dc.subject	Marine pathogen bacteria	es
dc.subject	Gilthead seabream (Sparus aurata)	es
dc.subject	Aquaculture.	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología	es
dc.title	In vitro effects of a natural marine algae polysaccharide (λ-carrageenin) on seabream erythrocytes, tumour cell lines and marine bacterial pathogens	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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