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Laser capture microdissection: Big data from small samples

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dc.contributor.authorDatta, Soma
dc.contributor.authorMalhotra, Lavina
dc.contributor.authorDickerson, Ryan
dc.contributor.authorChaffee, Scott
dc.contributor.authorSen, Chandan K.
dc.contributor.authorRoy, Sashwati
dc.date.accessioned2021-02-10T09:38:41Z
dc.date.available2021-02-10T09:38:41Z
dc.date.issued2015
dc.description.abstractAny tissue is made up of a heterogeneous mix of spatially distributed cell types. In response to any (patho) physiological cue, responses of each cell type in any given tissue may be unique and cannot be homogenized across cell-types and spatial co-ordinates. For example, in response to myocardial infarction, on one hand myocytes and fibroblasts of the heart tissue respond differently. On the other hand, myocytes in the infarct core respond differently compared to those in the peri-infarct zone. Therefore, isolation of pure targeted cells is an important and essential step for the molecular analysis of cells involved in the progression of disease. Laser capture microdissection (LCM) is powerful to obtain a pure targeted cell subgroup, or even a single cell, quickly and precisely under the microscope, successfully tackling the problem of tissue heterogeneity in molecular analysis. This review presents an overview of LCM technology, the principles, advantages and limitations and its down-stream applications in the fields of proteomics, genomics and transcriptomics. With powerful technologies and appropriate applications, this technique provides unprecedented insights into cell biology from cells grown in their natural tissue habitat as opposed to those cultured in artificial petri dish conditions.es
dc.formatapplication/pdfes
dc.format.extent15es
dc.identifier.citationHistology and Histopathology, vol. 30, nÂş 11, (2015)
dc.identifier.doi10.14670/HH-11-622
dc.identifier.issn1699-5848
dc.identifier.issn0213-5434
dc.identifier.urihttp://hdl.handle.net/10201/103001
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de BiologĂ­a Celular e HistologĂ­aes
dc.relationSin financiaciĂłn externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectLaser capture microdissection (LCM)es
dc.subjectGenomicses
dc.subjectProteomicses
dc.subjectTranscriptomicses
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - PatologĂ­a. Medicina clĂ­nica. OncologĂ­aes
dc.titleLaser capture microdissection: Big data from small sampleses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.30,nÂş11 (2015)