Production and localization of hydrogen peroxide and nitric oxide in grapevine cells elicited with cyclodextrins and methyl jasmonate

Abellán Rubio, Marina; Almagro Romero, Lorena; Belchí Navarro, Sarai; Pedreño García, María Ángeles

Publication:
Production and localization of hydrogen peroxide and nitric oxide in grapevine cells elicited with cyclodextrins and methyl jasmonate

dc.contributor.author	Abellán Rubio, Marina
dc.contributor.author	Almagro Romero, Lorena
dc.contributor.author	Belchí Navarro, Sarai
dc.contributor.author	Pedreño García, María Ángeles
dc.contributor.department	Biología Vegetal
dc.date.accessioned	2025-01-29T16:04:10Z
dc.date.available	2025-01-29T16:04:10Z
dc.date.issued	2019-06
dc.description	© 2019 Published by Elsevier GmbH. This document is the Published Manuscript version of a Published Work that appeared in final form in Journal of Plant Physiology. To access the final edited and published work see https://doi.org/10.1016/j.jplph.2019.03.013
dc.description.abstract	The use of methyl jasmonate, alone or in combination with cyclic oligosaccharides such as cyclodextrins, has proved to be a successful strategy for increasing the production of trans-resveratrol in Vitis vinifera cell cultures. However, understanding the intracellular signalling pathways involved in its production would improve the management of grapevine cells as biofactories of this high-value natural product. The results obtained herein conﬁrm the involvement of hydrogen peroxide and nitric oxide in cyclodextrins and methyl jasmonate-induced trans-resveratrol production in grapevine cell cultures. In fact, methyl jasmonate led to maximal intracellular levels of hydrogen peroxide and nitric oxide after 24 h of treatment, but extracellular hydrogen peroxide was only detected in the culture medium when grapevine cells were treated with cyclodextrins. The results derived from the cytochemical detection of H2O2 in elicited grapevine cell cultures also suggested that the combined treatment with cyclodextrins and methyl jasmonate not only increased the production of H2O2 but also released cell wall fragments with electron-dense deposits. Moreover, nitric oxide was localized in all the cellular compartments, particularly in the nucleus and cyto-plasmic organelles, whereas hydrogen peroxide was mainly found in cytoplasmic areas close to the cell wall, and in the nucleoplasm.	es
dc.format	application/pdf	es
dc.format.extent	7	es
dc.identifier.citation	Journal of Plant Physiology, 2019, Vol. 237, pp. 80-86
dc.identifier.doi	https://doi.org/10.1016/j.jplph.2019.03.013
dc.identifier.issn	Print: 0176-1617
dc.identifier.issn	Electronic: 1618-1328
dc.identifier.uri	http://hdl.handle.net/10201/149662
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	This work has been supported by the Ministerio de Economía y Competitividad (No. BIO2017-82374-R) and Fundación Seneca-Agencia de Ciencia y Tecnología de la Región de Murcia (No. 19876/GERM/15).	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S0176161719300495	es
dc.rights.accessRights	info:eu-repo/semantics/restrictedAccess
dc.subject	Cyclodextrins	es
dc.subject	Grapevine cell cultures	es
dc.subject	Hydrogen peroxide	es
dc.subject	Methyl jasmonate	es
dc.subject	Nitric oxide	es
dc.subject	Trans resveratrol	es
dc.title	Production and localization of hydrogen peroxide and nitric oxide in grapevine cells elicited with cyclodextrins and methyl jasmonate	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
relation.isAuthorOfPublication	26b10f99-26d5-4ed0-a830-5b27d6665ae6
relation.isAuthorOfPublication	4ab095a1-4184-489e-82f9-cf1cb3bc8264
relation.isAuthorOfPublication	6a60e21b-276a-41c1-9f7e-16b7c933da77
relation.isAuthorOfPublication.latestForDiscovery	26b10f99-26d5-4ed0-a830-5b27d6665ae6