Publication: Histological repair of damaged spinal cord tissue
from chronic contusion injury of rat: A LM observation
Authors
Zhang, Shu-Xin ; Huang, Fengfa ; Gates, Mary ; White, Jason ; Holmberg, Eric G.
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Publisher
Murcia: F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The spinal cord has an intrinsic, limited
ability of spontaneous repair; the endogenous repair of
damaged tissue starts a few days after spinal cord injury
(SCI). To date, however, detailed observation in
histology at the injury site has not been well
documented. In the present study we analyzed the
histological structure of the repaired tissue from injury
site of rats 6 or 14 weeks after contusion injury (NYU
impactor device, 25 mm height setting) on T10, and rats
8 weeks after transplantation of lamina propria (LP) or
acellular lamina propria. We found that the initial
repaired tissue can be histologically divided into three
different zones, i.e., fibrotic, cellular and axonal. The
fibrotic zone consists of invading connective tissue,
while the cellular zone is composed of invading, densely
compacted Schwann cells. Schwann cells migrate from
dorsal roots laterally toward and merge underneath the
fibrotic zone, forming the U-shape shell of the cellular
zone. The major component of the axonal zone is
regenerating axons. Schwann cells myelinate
regenerating axons in all three zones. In rats with
combination treatments including scar ablation and LP
transplantation, both cellular and axonal zones
significantly expand in size, resulting in the
disappearance of the lesion cavity and the integration of
repaired tissue with spared tissue. Olfactory ensheathing
cells from transplanted LP may promote the expansion
of the cellular and axonal zones through stimulating host
Schwann cells, indirectly contributing to tissue repair
and axonal regeneration. The ependyma-derived cells
may be directly involved in tissue repair, but not
contribute to the formation of myelin sheaths.
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