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Nerve fibre layer degeneration and retinal ganglion cell loss long term after optic nerve crush or transection in adult mice

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dc.contributor.authorSánchez-Migallón Carreras, María del Cielo
dc.contributor.authorValiente Soriano, Francisco Javier
dc.contributor.authorSalinas Navarro, Manuel Ángel
dc.contributor.authorNadal-Nicolás, Francisco Manuel
dc.contributor.authorJiménez-López, M.
dc.contributor.authorVidal Sanz, Manuel
dc.contributor.authorAgudo Barriuso, Marta
dc.contributor.departmentOftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
dc.contributor.otherFacultad de Medicina
dc.date.accessioned2026-01-27T19:48:52Z
dc.date.available2026-01-27T19:48:52Z
dc.date.copyright© 2018 The Authors
dc.date.issued2018-02-13
dc.description.abstractWe have investigated the long term effects of two different models of unilateral optic nerve (ON) lesion on retinal ganglion cells (RGCs) and their axons, in the injured and contralateral retinas of adult albino mice. Intact animals were used as controls. The left ON was intraorbitally crushed or transected at 0.5 mm from the optic disk and both retinas were analyzed at 2, 3, 5, 7, 14, 30, 45 or 90 days after injury. RGCs were immunoidentified with anti-Brn3a, and their axons with anti-highly phosphorylated axonal neurofilament subunit H (pNFH). After both lesions, RGC death in the injured retinas is first significant at day 3, and progresses quickly up to 7 days slowing down till 90 days. In the same retinas, the anatomical loss of RGC axons is not evident until day 30. However, by two days after both lesions there are changes in the expression pattern of pNFH: axonal beads, axonal club- or bulb-like formations, and pNFH+RGC somas. The number of pNFH+RGC somata peak at day 5 after either lesion and is significantly higher than in intact retinas at all time points. pNFH+RGC somata are distributed across the retina, in accordance with the pattern of RGC death which is diffuse and homogenous. In the contralateral retinas there is no RGC loss, but there are few pNFH+RGCs from day 2 to day 90. In conclusion, in albino mice, axotomy-induced RGC death precedes the loss of their intraretinal axons and occurs in two phases, a rapid and a slower, but steady, one. Injured retinas show similar changes in the pattern of pNFH expression and a comparable course of RGC loss
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dc.format.extent11
dc.identifier.citationExperimental Eye Research 170 (2018) 40–50
dc.identifier.doihttps://doi.org/10.1016/j.exer.2018.02.010
dc.identifier.issn0014-4835
dc.identifier.urihttp://hdl.handle.net/10201/195330
dc.languageeng
dc.publisherElsevier
dc.relationThe Spanish Ministry of Economy and Competitiveness, Instituto de Salud Carlos III, Fondo Europeo de Desarrollo Regional “Una Manera de Hacer Europa” (SAF2015-67643-P, PI16/00031), and Fundación Séneca, Agencia de Ciencia y Tecnología Región de Murcia (19881/GERM/15).
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S001448351730619X
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectNerve fiber layer
dc.subjectOptic nerve crush
dc.subjectOptic nerve transection
dc.subjectBrn3a
dc.subjectAntibodies antineurofilaments
dc.subjectAdult albino mice
dc.subjectAdult albino mice Retinal nerve fiber layer
dc.subjectRetrograde axonal degeneration
dc.subjectAxotomy
dc.subjectRetinal neurodegeneration
dc.subject.odsObjetivo 3: Salud
dc.titleNerve fibre layer degeneration and retinal ganglion cell loss long term after optic nerve crush or transection in adult mice
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
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