Publication: Chronic ethanol feeding alters the epithelial cell proliferation and apoptosis in rat gastric mucosa
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Date
2007
Authors
Ge, Y. B. ; Du, J. ; Fan, L.L. ; Li, Y.C. ; Gu, L.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
We developed a chronic drinking rat model
to investigate the long-term effects of ethanol feeding on
cell proliferation and apoptosis in rat stomach. Adult
male Sprague-Dawley (SD) rats received either an
isocaloric control or drinking water containing 6% (v/v)
ethanol as their only water intake for 1, 3, 7, 14 and 28
days. At the end of each feeding period, animals were
sacrificed and the stomach was dissected for the sample
preparation. The cell proliferation and apoptosis in
gastric mucosa of rats in different groups were analyzed
by flow cytometer, immunohistochemistry and computer
image analysis. In the flow cytometric study, compared
with the control, the cell apoptosis in gastric mucosa of
the rats was enhanced during the exposure to the ethanol
in 3rd to 28th day. Otherwise the cell proliferation was
increased in 3rd to 14th days, and decreased in 28th
days, respectively. The results were confirmed by
immunohistochemistry and computer image analysis
studied. This finding suggested that short-term chronic
adequate alcohol intake may enhance the cell turnover of gastric mucosa. Long-term stimulus with the low
concentration ethanol may cause the impairment of the
cell turnover function of the gastric mucosa and may be
one of the mechanisms underlying the gastric pathology
associated with alcohol abuse.
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