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Reg I protein in healthy and seminoma human testis

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dc.contributor.authorMauro, V.es
dc.contributor.authorCarette, D.es
dc.contributor.authorChevallier, D.
dc.contributor.authorMichiels, J.F.
dc.contributor.authorSegretain, D.
dc.contributor.authorPointis, G.
dc.contributor.authorSénégas-Balas, F.
dc.date.accessioned2013-01-22T10:04:06Z
dc.date.available2013-01-22T10:04:06Z
dc.date.issued2008
dc.description.abstractRegenerating gene (Reg), encodes a secretory protein with growth and differentiation stimulating effects mostly in digestive tissues. Overexpression of Reg proteins and specifically of Reg I, one member of the Reg family, is associated with several human diseases and cancers. In the present study we analyzed the expression of Reg I in normal rodent and human testes where germ cells normally proliferate and differentiate into spermatozoa, and in seminoma testis, the most common cancer of young men. Western blot analyses demonstrated the presence of a specific band at 19 kDa in human and rodent testis extracts. Immunofluorescence and deconvolution microscopy demonstrated that Reg I was present within the seminiferous tubules in both Sertoli and germ cells. By using a Sertoli cell line we demonstrated that Reg I was localized at the plasma membrane even in the absence of contact between neighboring cells and appeared before the tight junction associated protein ZO-1 was revealed at this location. Reg I was strongly expressed in human seminoma testis tissue and in a human tumor germ cell line where the immunoreactive signal was mainly detected at the plasma membrane level. These data showing for the first time the weak presence of Reg I in the normal testis and its strong expression in the testis cancer suggest a potential role of Reg I in normal and neoplastic germ cell proliferation.es
dc.formatapplication/pdfes
dc.format.extent9es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/29847
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectTestises
dc.subjectSeminomaes
dc.subject.other611 - Anatomíaes
dc.titleReg I protein in healthy and seminoma human testises
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.23,nº10 (2008)