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Inherited photoreceptor degeneration causes the death of melanopsin-positive retinal ganglion cells and increases their coexpression of Brn3a

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dc.contributor.authorGarcía Ayuso, Diego
dc.contributor.authorDi Pierdomenico, Johnny
dc.contributor.authorEsquiva, Gema
dc.contributor.authorNadal-Nicolás, Francisco Manuel
dc.contributor.authorPinilla, Isabel
dc.contributor.authorCuenca, Nicolás
dc.contributor.authorVidal Sanz, Manuel
dc.contributor.authorAgudo Barriuso, Marta
dc.contributor.authorVillegas Pérez, Maria Paz
dc.contributor.departmentOftalmología, Optometría, Otorrinolaringología y Anatomía Patológica
dc.contributor.otherFacultades de la UMU::Facultad de Medicina
dc.date.accessioned2026-01-27T11:12:33Z
dc.date.available2026-01-27T11:12:33Z
dc.date.copyright© 2015 The Association for Research in Vision and Ophthalmology, Inc.
dc.date.issued2015-07-20
dc.description.abstractPurpose: To study the population of intrinsically photosensitive retinal ganglion cells (melanopsin-expressing RGCs, m+RGCs) in P23H-1 rats, a rat model of inherited photoreceptor degeneration. Methods: At postnatal (P) times P30, P365, and P540, retinas from P23H dystrophic rats (line 1, rapid degeneration; and line 3, slow degeneration) and Sprague Dawley (SD) rats (control) were dissected as whole-mounts and immunodetected for melanopsin and/or Brn3a. The dendritic arborization of m+RGCs and the numbers of Brn3a+RGCs and m+RGCs were quantified and their retinal distribution and coexpression analyzed. Results: In SD rats, aging did not affect the population of Brn3a+RGCs or m+RGCs or the percentage that showed coexpression (0.27%). Young P23H-1 rats had a significantly lower number of Brn3a+RGCs and showed a further decline with age. The population of m+RGCs in young P23H-1 rats was similar to that found in SD rats and decreased by 22.6% and 28.2% at P365 and P540, respectively, similarly to the decrease of the Brn3a+RGCs. At these ages the m+RGCs showed a decrease of their dendritic arborization parameters, which was similar in both the P23H-1 and P23H-3 lines. The percentage of coexpression of Brn3a was, however, already significantly higher at P30 (3.31%) and increased significantly with age (10.65% at P540). Conclusions: Inherited photoreceptor degeneration was followed by secondary loss of Brn3a+RGCs and m+RGCs. Surviving m+RGCs showed decreased dendritic arborization parameters and increased coexpression of Brn3a and melanopsin, phenotypic and molecular changes that may represent an effort to resist degeneration and/or preferential survival of m+RGCs capable of synthesizing Brn3a.
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dc.identifier.citationInvest Ophthalmol Vis Sci. 2015 Jul;56(8):4592-604.
dc.identifier.doihttps://doi.org/10.1167/iovs.15-16808
dc.identifier.eissn1552-5783
dc.identifier.issn0146-0404
dc.identifier.urihttp://hdl.handle.net/10201/194549
dc.languageeng
dc.publisherAssociation for Research in Vision and Ophthalmology
dc.relationSupported by grants from the Spanish Ministry of Economy and Competitiveness: SAF-2012-38328; ISCIII-FEDER ‘‘Una manera de hacer Europa’’ PI13/00643, PI13/01266, and BFU2012-36845, RETICS: RD12/0034/0014, and RD12/0034/0010.
dc.relation.publisherversionhttps://iovs.arvojournals.org/article.aspx?articleid=2398229
dc.rightsAttribution 4.0 International*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectP23H
dc.subjectRetinitis pigmentosa
dc.subjectMelanopsin
dc.subjectBrn3a
dc.subjectAutomated quantification
dc.subjectIntrinsically photosensitive
dc.subjectPhotoreceptor degeneration
dc.subjectIntrinsically photosensitive RGCs
dc.subject.odsObjetivo 3: Salud
dc.titleInherited photoreceptor degeneration causes the death of melanopsin-positive retinal ganglion cells and increases their coexpression of Brn3a
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
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