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Fibroblast remodeling of adsorbed collagen type IV is altered in contact with cancer cells

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dc.contributor.authorManeva-Radicheva, L.es
dc.contributor.authorEbert, U.es
dc.contributor.authorDimoudis, N.
dc.contributor.authorAltankov, G.
dc.date.accessioned2013-01-18T13:35:26Z
dc.date.available2013-01-18T13:35:26Z
dc.date.issued2008
dc.description.abstractA series of co-culture experiments between fibroblasts and H-460 human lung carcinoma cells were performed to learn more about the fate of adsorbed type IV collagen (Coll IV). Fibroblasts were able to spatially rearrange Coll IV in a specific linear pattern, similar but not identical to the fibronectin (FN) fibrils. Coll IV partly co-aligns with fibroblast actin cytoskeleton and transiently co-localize with FN, as well as with ß1 and a2 integrin clusters, suggesting a cell-dependent process. We further found that this Coll IV reorganization is suppressed in contact with H460 cells. Zymography revealed strongly elevated MMP-2 activity in supernatants of co-cultures, but no activity when fibroblasts or cancer cells were cultured alone. Thus, we provide evidence that reorganization of substrate associated Coll IV is a useful morphological approach for in vitro studies on matrix remodeling activity during tumorigenesis.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/29709
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectFibroblastses
dc.subjectCancer cellses
dc.subject.other616 - Patología. Medicina clínica. Oncologíaes
dc.titleFibroblast remodeling of adsorbed collagen type IV is altered in contact with cancer cellses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.23, nº7 (2008)