Publication: Temporal and spatial distribution of TGF-B isoforms and signaling intermediates in corneal regenerative wound repair
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Date
2009
Authors
Huh, Man-IL ; Chang, Yongmin ; Jung, Jae-Chang
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The present study analyzed the temporal and
spatial expression of TGF-ß isoforms and activated
pSmad2 and p38MAPK during epithelial debridement
wound repair, using chick cornea by immunohistochemistry.
Normal corneas showed low-level TGFßs
staining. Following wounding, TGF-ß1 expression
was strong in the Bowman’s layer (BL). TGF-ß3
expression was confined to basal cells in the
regenerating and unwounded regions, and was not
detected in migrating epithelial, stromal or endothelial
cells. In addition, TGF-ß3 treatment stimulated the
proliferation of cultured epithelial cells. Our present
findings seem to suggest that the TGF-ß3 signal may be
required for epithelial cell proliferation. TGF-ß2
expression was strong in migrating and proliferating
epithelial cells, many active migrating fibroblasts at the
wound edge, endothelial cells and Descemet’s
membrane (DM). Although both nuclear pSmad2 and
p38MAPK staining was observed in many basal
epithelial cells, pSmad2 positive cells were co-localized
with PCNA positive cells. Therefore, it seems likely that
the pSmad2 signal may affect epithelial cell proliferation
in healing corneas. Both pSmad2 and p38MAPK
expression were also observed in endothelial cells.
Interestingly, many active fibroblasts over the whole
stroma in early wound healing at day 2 expressed
nuclear pSmad2, but little if any cytoplasmic p38MAPK.
Collectively, temporal/spatial up-regulation and
distribution of the three TGF-ß isoforms, as well as
concerted activation of both Smad2 and p38MAPK,
appears to be a key aspect of regenerative corneal wound
healing in the chick.
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