Publication: Estimation and comparison of the
contents of blood group B antigens in
selected human tissues by microphotometric
quantification of Griffonia simplicifolia agglutinin 1-B,
staining with or without prior a-galactosidase digestion
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Date
1997
Authors
Ito, N. ; Nagaike, C. ; Morimura, Y. ; Hatake, K.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Griffonia simplicifolia agglutinin 1-B4
(GSAI-B4) has broader specificity for B antigen variants
and can recognize the antigens in a wide variety of
human tissues. Thus, the concentration range of GSAIB4
required for staining and the susceptibility of staining
to a-galactosidase digestion is presumed to correlate
well with the density of B antigens in tissue sections. By
microphotometric quantification of staining intensity at
different concentrations of GSAI-B4 with or without agalactosidase
digestion, concentration of B antigens in
selected tissues was evaluated and compared. Based on
the present results and the previous ones of direct
measurement of galactose of B antigens in sublingual
glands and red blood cells (Ito et al., 1993), the order of
concentration of B antigens in tissues examined was
estimated as follows; mucous cells of sublingual glands
from German nonsecretors < red blood cells and
vascular endothelial cells (=2.7x10-3nmole/cm2), thyroid
papillary carcinomas and Hassall's corpuscles from
nonsecretors < mucous cells of sublingual gland from
Japanese nonsecretors < pancreatic acinar cells from
both secretor and nonsecretors, Hassall's corpuscles and
kidney collecting tubules form secretors < mucous cells
of sublingual gland from secretors (>8.5-11.7
nmole/cm2) and mucous cells of Brunner 'S gland from
nonsecretors < mucous cells of Brunner's gland from
secretors. From the above estimation, it is apparent that
the expression of B antigen in Brumer's gland is partly
dependent on the secretor status of individuals and that
Japanese nonsecretors secrete substantial amounts of B
antigens from sublingual gland while German nonsecretors
do not. The present results also revealed an
unexpected staining behavior of GSAI-B4 in some
tissues, i.e. in mucous cells of sublingual glands and
collecting tubules of kidney from secretors, staining
olrprint reguests to: Nobuaki Ito, Ph.D., Department of Legal Medicine,
Nara Medical University, Kashihara Nara 834, Japan
intensity was markedly depressed at higher
concentration of the lectin and this depression was
recovered by prior a-galactosidase digestion. In
addition, the present method was successfully applied
for the estimation of the content of B antigens neoexpressed
in thyroid papillary carcinomas, showing that
the content of B antigen had a similar leve1 to that of red
blood cells and vascular endothelial cells.
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