Publication: Gut region-specific rearrangement of the cellular and
subcellular compartments of nitric oxide synthase
isoforms after chronic ethanol consumption in rats
Authors
Bódi, Nikolett ; Jancsó, Zsanett ; Talapka, Petra ; Pál, Alexandra ; Poles, Marietta Zita ; Bagyánszki, Mária ; Hermesz, Edit ; Fekete, Éva
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
https://doi.org/10.14670/HH-29.1547
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info:eu-repo/semantics/article
Description
Abstract
We recently provided evidence of cell-typespecific differences in the subcellular distributions of the
three nitric oxide synthase (NOS) isoforms in the
myenteric neurons, enteric smooth muscle cells and the
capillary endothelium of the rat duodenum. We
hypothesized that the presence of three NOS isoforms in
the same type of cells with differences in subcellular
compartmentalization might reflect a functional
plasticity. Therefore, investigation of the possible
rearrangement of cellular and subcellular NOS
compartments in different gut segments following
chronic ethanol treatment was the aim of this study.
Rats were randomly divided into two groups and
received water or 20% ethanol solution, preceded by
short periods of adaptation with 10% and 15% ethanol.
After 8 weeks, segments of duodenum, ileum and colon
of the control and the alcohol-treated rats were processed
for post-embedding immunohistochemistry and RT-PCR.
The quantitative differences in the numbers of gold
particles indicative of the different NOSs and their
relative mRNA levels between the two experimental
groups varied greatly, depending on the gut segment, and
also on the cellular and subcellular compartments
investigated. The chronic ethanol administration had the
opposite effect on the quantitative distribution of the
neuronal and endothelial NOS labelling gold particles in
the different cellular compartments and resulted in
subcellular rearrangement of NOS labels along the
gastrointestinal tract.
The intestinal region-specific rearrangement of the
cellular and subcellular NOS compartments may
possibly result in functional plasticity and help to
maintain the optimum NO level under pathological
conditions.
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Citation
Histology and Histopathology, Vol. 29, n.º 12 (2014)
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