Publication:
LncRNA UBE2R2-AS1, as prognostic marker, promotes cell proliferation and EMT in prostate cancer

Thumbnail Image
Files
Wang-38-637-645-2023.pdf(5.52 MB)
Date
2023
relationships.isAuthorOfPublication
relationships.isSecondaryAuthorOf
relationships.isDirectorOf
Authors
Wang, Feng ; Zhao, Miao ; Jiang, Yuehong ; Xia, Silong ; Sun, Dapeng ; Zhou, Dahong ; Dong, Zipu
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
publication.page.editor
publication.page.department
DOI
https://doi.org/10.14670/HH-18-505
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Background. Long noncoding RNA ubiquitin-conjugating enzyme E2 R2 antisense RNA 1 (UBE2R2-AS1) has been recently reported to participate in the progression of tumors, including glioma and liver cancer. However, the roles of UBE2R2-AS1 in prostate cancer (PC) remained poorly understood. Methods. The expression of UBE2R2-AS1 was determined in tumor tissues and paired adjacent tissues from PC patients using quantitative reverse transcription PCR analysis. Correlation between UBE2R2-AS1 expression and clinicopathological parameters and overall survival were investigated by Chi-square test and Kaplan-Meier method analysis. The in vitro experiments, including CCK-8 assay, colony formation, flow cytometry and transwell assay were performed to investigate the functional role of UBE2R2-AS1 knockdown or overexpression on PC cell lines (PC-3 and DU145). Related protein expression levels were measured by western blot analysis. Results. Our data showed that UBE2R2-AS1 expression was significantly upregulated in PC tissues compared with that in adjacent tissues. The high levels of UBE2R2-AS1 were associated with high Gleason score, advanced clinical T stage, lymph node metastasis and poor prognosis. Knockdown of UBE2R2-AS1 suppressed cell proliferation, migration and invasion, induced cell cycle G0/G1 arrest and apoptosis in PC cells, along with decreased expression of PCNA, CDK4, Cyclin D1, Bcl-2, N-cadherin and Vimentin, and increased E-cadherin expression. Overexpression of UBE12R2-AS1 obtained the opposite results in PC cells. Conclusions. Our findings suggest that UBE2R2- AS1 might be a potential diagnostic and/or therapeutic target in PC.
publication.page.subject
Prostate cancer , UBE2R2-AS1 , Prognosis , Proliferation , Migration
Citation
Histology and Histopathology Vol. 38, nº6 (2023)
URI
http://hdl.handle.net/10201/131387
item.page.embargo
Collections
Histology and histopathology Vol.38, nº6 (2023)
Ir a Estadísticas