Population genomics and phylogeography of a clonal bryophyte with spatially separated sexes and extreme sex ratios

Villarreal A., Juan Carlos; McFarland, Kenneth; Goffinet, Bernard; Alonso García, Marta

Publication:
Population genomics and phylogeography of a clonal bryophyte with spatially separated sexes and extreme sex ratios

dc.contributor.author	Villarreal A., Juan Carlos
dc.contributor.author	McFarland, Kenneth
dc.contributor.author	Goffinet, Bernard
dc.contributor.author	Alonso García, Marta
dc.contributor.department	Biología Vegetal
dc.date.accessioned	2025-01-17T09:53:18Z
dc.date.available	2025-01-17T09:53:18Z
dc.date.issued	2020-05-08
dc.description	© 2020 Alonso-García, Villarreal A, McFarland and Goffinet. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in Frontiers in Plant Science. To access the final edited and published work see https://doi.org/10.3389/fpls.2020.00495
dc.description.abstract	The southern Appalachian (SA) is one of the most biodiversity−rich areas in North America and has been considered a refugium for many disjunct plant species, from the last glacial period to the present. Our study focuses on the SA clonal hornwort, Nothoceros aenigmaticus J. C. Villarreal & K. D. McFarland. This hornwort was described from North Carolina and is widespread in the SA, growing on rocks near or submerged in streams in six and one watersheds of the Tennessee (TR) and Alabama (AR) Rivers, respectively. Males and female populations occur in different watersheds, except in the Little Tennessee (TN) River where an isolated male population exists ca. 48 km upstream from the female populations. The sex ratio of 1:0 seems extreme in each population. In this study, we use nuclear and organellar microsatellites from 250 individuals from six watersheds (seven populations) in the SA region and two populations from Mexico (23 individuals). We, then, selected 86 individuals from seven populations and used genotyping by sequencing to sample over 600 bi-allelic markers. Our results suggest that the SA N. aenigmaticus and Mexican plants are a nested within a clade of sexual tropical populations. In the US populations, we confirm an extreme sex ratio and only contiguous US watersheds share genotypes. The phylogenetic analysis of SNP data resolves four clusters: Mexican populations, male plants (Little Pigeon and Pigeon river watersheds) and two clusters of female plants; one from the Little Tennessee and Hiwassee Rivers (TR) and the other from the Ocoee (TR) and Coosa (AR) Rivers. All clusters are highly differentiated (Fst values over 0.9). In addition, our individual assignment analyses and PCAs reflect the phylogenetic results grouping the SA samples in three clades and recovering males and female plants with high genetic differentiation (Fst values between 0.5 and 0.9 using microsatellites and bi-allelic markers). Our results point to Pleistocene events shaping the biogeographical pattern seen in US populations. The extreme sex ratio reflects isolation and highlights the high vulnerability of the populations in the SA.	es
dc.format	application/pdf	es
dc.format.extent	15	es
dc.identifier.citation	Frontiers in Plant Science 11:495
dc.identifier.doi	https://doi.org/10.3389/fpls.2020.00495
dc.identifier.issn	Electronic: 1664-462X
dc.identifier.uri	http://hdl.handle.net/10201/148669
dc.language	eng	es
dc.publisher	Frontiers Media	es
dc.relation	Financial support came from the NSF DDIG-0910258 award to JV and the Ronald Bamford Endowment (UCONN), the UCONN president Fellowship and the NSERC Discovery Grant (CRNSG- RGPIN/05967-2016). MA-G benefited from financial support from “Fundación Séneca-Agencia de Ciencia y Tecnología de la Región de Murcia, España” (project 20369/PD/17).	es
dc.relation.publisherversion	https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00495/full	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Atribución 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	Clonality	es
dc.subject	Hornwort
dc.subject	Nothoceros
dc.subject	Population genomic
dc.subject	Sex separation
dc.subject	SNPs
dc.subject	Stacks
dc.title	Population genomics and phylogeography of a clonal bryophyte with spatially separated sexes and extreme sex ratios	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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